ARG45642
anti-RNF25 antibody
anti-RNF25 antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes RNF25 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | FACS, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | RNF25 |
| 抗原物种 | Human |
| 抗原 | Recombinant protein containing to human RNF25. |
| 偶联标记 | Un-conjugated |
| 別名 | RNF25; Ring Finger Protein 25; AO7; E3 Ubiquitin-Protein Ligase RNF25; FLJ13906; RING-Type E3 Ubiquitin Transferase RNF25; Ring Finger Protein AO7; RING Finger Protein AO7; RING Finger Protein 25; EC 2.3.2.27 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 实际分子量 | 62 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified |
| 缓冲液 | 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose. |
| 稳定剂 | 4% Trehalose |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | RNF25 |
| 全名 | Ring Finger Protein 25 |
| 背景介绍 | The protein encoded by this gene contains a RING finger motif. The mouse counterpart of this protein has been shown to interact with Rela, the p65 subunit of NF-kappaB (NFKB), and modulate NFKB-mediated transcription activity. The mouse protein also binds ubiquitin-conjugating enzymes (E2s) and is a substrate for E2-dependent ubiquitination. [provided by RefSeq, Jul 2008] |
| 生物功能 | E3 ubiquitin-protein ligase that plays a key role in the RNF14-RNF25 translation quality control pathway, a pathway that takes place when a ribosome has stalled during translation, and which promotes ubiquitination and degradation of translation factors on stalled ribosomes. [UniProt] |
| 细胞定位 | Cytoplasm. [UniProt] |
| 预测分子量 | 51 kDa |
| 翻译后修饰 | Phosphoprotein; Ubl conjugation. [UniProt] |
检测图片 (6) Click the Picture to Zoom In
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ARG45642 anti-RNF25 antibody IHC-P image
Immunohistochemistry: Human lung adenocarcinoma stained with ARG45642 anti-RNF25 antibody at 2 μg/ml dilution.
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ARG45642 anti-RNF25 antibody WB image
Western blot: Hela and U251 stained with ARG45642 anti-RNF25 antibody at 0.5 μg/ml dilution.
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ARG45642 anti-RNF25 antibody FACS image
Flow Cytometry: 293T stained with ARG45642 anti-RNF25 antibody at 1 µg/10^6 cells dilution.
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ARG45642 anti-RNF25 antibody IHC-P image
Immunohistochemistry: Rat testis stained with ARG45642 anti-RNF25 antibody at 2 μg/ml dilution.
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ARG45642 anti-RNF25 antibody WB image
Western blot: Rat testis stained with ARG45642 anti-RNF25 antibody at 0.5 μg/ml dilution.
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ARG45642 anti-RNF25 antibody WB image
Western blot: Mouse testis stained with ARG45642 anti-RNF25 antibody at 0.5 μg/ml dilution.
