ARG63302
anti-RNF34 / RFI antibody
anti-RNF34 / RFI antibody for IHC-Formalin-fixed paraffin-embedded sections and Human
Cell Biology and Cellular Response antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes RNF34 / RFI |
|---|---|
| 反应物种 | Hu |
| 预测物种 | Ms, Rat, Cow, Dog |
| 应用 | IHC-P |
| 特异性 | This antibody is expected to recognize both reported isoforms (NP_919247.1 and NP_079402.2). |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | RNF34 / RFI |
| 抗原物种 | Human |
| 抗原 | KAGATSMWASCC |
| 偶联标记 | Un-conjugated |
| 別名 | CARP-1; E3 ubiquitin-protein ligase RNF34; hRFI; EC 6.3.2.-; Human RING finger homologous to inhibitor of apoptosis protein; RING finger protein RIFF; Caspases-8 and -10-associated RING finger protein 1; FYVE-RING finger protein Momo; RIFF; Caspase regulator CARP1; RIF; RFI; RING finger protein 34; CARP1 |
应用说明
| 应用建议 |
|
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|---|---|---|---|---|---|
| 应用说明 | IHC-P: Antigen Retrieval: Steam tissue section in Tris/EDTA buffer (pH 9.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by antigen affinity chromatography. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 背景介绍 | The protein encoded by this gene contains a RINF finger, a motif known to be involved in protein-protein and protein-DNA interactions. This protein interacts with DNAJA3/hTid-1, which is a DnaJ protein reported to function as a modulator of apoptosis. Overexpression of this gene in Hela cells was shown to confer the resistance to TNF-alpha induced apoptosis, suggesting an anti-apoptotic function of this protein. This protein can be cleaved by caspase-3 during the induction of apoptosis. This protein also targets p53 and phospho-p53 for degradation. Alternatively splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Feb 2012] |
| 研究领域 | Cell Biology and Cellular Response antibody |
| 预测分子量 | 42 kDa |
| 翻译后修饰 | Autoubiquitinated (in vitro). Proteolytically cleaved by caspases upon induction of apoptosis by TNF. |
检测图片 (1) Click the Picture to Zoom In
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ARG63302 anti-RNF34 / RFI antibody IHC-P image
Immunohistochemistry: paraffin embedded Human Kidney. (Steamed antigen retrieval with Tris/EDTA buffer pH 9) stained with ARG63302 anti-RNF34 / RFI antibody at 4 µg/ml dilution followed by HRP-staining.
