ARG63564

anti-RNF8 antibody

anti-RNF8 antibody for Flow cytometry,Western blot and Human

Cell Biology and Cellular Response antibody; Gene Regulation antibody

概述

产品描述 Goat Polyclonal antibody recognizes RNF8
反应物种 Hu
应用 FACS, WB
特异性 This antibody is expected to recognize both reported isoforms (NP_003949.1 and NP_898901.1).
宿主 Goat
克隆 Polyclonal
同位型 IgG
靶点名称 RNF8
抗原物种 Human
抗原 GEPGFFVTGDRAG-C
偶联标记 Un-conjugated
別名 E3 ubiquitin-protein ligase RNF8; RING finger protein 8; EC 6.3.2.-; hRNF8

应用说明

应用建议
应用 推荐稀释比
FACS10 µg/ml
WB0.5 - 2 µg/ml
应用说明 WB: Recommend incubate at RT for 1h.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Purified from goat serum by antigen affinity chromatography.
缓冲液 Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA.
抗菌剂 0.02% Sodium azide
稳定剂 0.5% BSA
浓度 0.5 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 9025 Human RNF8

Swiss-port # O76064 Human E3 ubiquitin-protein ligase RNF8

背景介绍 The protein encoded by this gene contains a RING finger motif and an FHA domain. This protein has been shown to interact with several class II ubiquitin-conjugating enzymes (E2), including UBE2E1/UBCH6, UBE2E2, and UBE2E3, and may act as an ubiquitin ligase (E3) in the ubiquitination of certain nuclear proteins. This protein is also known to play a role in the DNA damage response and depletion of this protein causes cell growth inhibition and cell cycle arrest. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2012]
研究领域 Cell Biology and Cellular Response antibody; Gene Regulation antibody
预测分子量 56 kDa
翻译后修饰 Autoubiquitinated through 'Lys-48' and 'Lys-63' of ubiquitin. 'Lys-63' polyubiquitination is mediated by UBE2N. 'Lys-29'-type polyubiquitination is also observed, but it doesn't require its own functional RING-type zinc finger.

检测图片 (2) Click the Picture to Zoom In

  • ARG63564 anti-RNF8 antibody WB image

    Western blot: 35 µg of HepG2 (A) and Jurkat (B) nuclear lysates (in RIPA buffer) stained with ARG63564 anti-RNF8 antibody at 1 µg/ml dilution and incubated at RT for 1 hour.

  • ARG63564 anti-RNF8 antibody FACS image

    Flow Cytometry: Paraformaldehyde-fixed HeLa cells permeabilized with 0.5% Triton. Cells were stained with ARG63564 anti-RNF8 antibody (blue line) at 10 µg/ml dilution for 1 hour, followed by incubation with Alexa FluorR 488 labelled secondary antibody. IgG control: Unimmunized goat IgG (black line).