ARG41323
anti-RSPO1 / R spondin 1 antibody
anti-RSPO1 / R spondin 1 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes RSPO1 / R spondin 1 |
|---|---|
| 反应物种 | Hu, Ms |
| 预测物种 | Rat |
| 应用 | IHC-P, WB |
| 特异性 | At least three isoforms of RSPO1 are known to exist. This antibody will detect the largest and smallest isoforms. RSPO1 antibody will not cross-react with other RSPO family members. |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | RSPO1 / R spondin 1 |
| 抗原物种 | Human |
| 抗原 | A 16 amino acid synthetic peptide within the first 50 amino acids of Human RSPO1. |
| 偶联标记 | Un-conjugated |
| 別名 | hRspo1; Roof plate-specific spondin-1; R-spondin-1; RSPO; CRISTIN3 |
应用说明
| 应用建议 |
|
||||||
|---|---|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | Human heart | ||||||
| 实际分子量 | ~ 26 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | PBS and 0.02% Sodium azide. |
| 抗菌剂 | 0.02% Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | RSPO1 |
| 全名 | R-spondin 1 |
| 背景介绍 | This gene encodes a secreted activator protein with two cysteine-rich, furin-like domains and one thrombospondin type 1 domain. The encoded protein is a ligand for leucine-rich repeat-containing G-protein coupled receptors (LGR proteins) and positively regulates the Wnt signaling pathway. In mice, the protein induces the rapid onset of crypt cell proliferation and increases intestinal epithelial healing, providing a protective effect against chemotherapy-induced adverse effects. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2014] |
| 生物功能 | Activator of the canonical Wnt signaling pathway by acting as a ligand for LGR4-6 receptors. Upon binding to LGR4-6 (LGR4, LGR5 or LGR6), LGR4-6 associate with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. Also regulates the canonical Wnt/beta-catenin-dependent pathway and non-canonical Wnt signaling by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. Acts as a ligand for frizzled FZD8 and LRP6. May negatively regulate the TGF-beta pathway. Has a essential roles in ovary determination. [UniProt] |
| 细胞定位 | Secreted. Nucleus. Note=Seems to mainly localize to nucleoli. [UniProt] |
| 预测分子量 | 29 kDa |
检测图片 (6) Click the Picture to Zoom In
-
ARG41323 anti-RSPO1 / R spondin 1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human spleen tissue stained with ARG41323 anti-RSPO1 / R spondin 1 antibody at 20 µg/ml dilution.
-
ARG41323 anti-RSPO1 / R spondin 1 antibody WB image
Western blot: Human heart lysate stained with ARG41323 anti-RSPO1 / R spondin 1 antibody at 1 and 2 µg/ml dilution.
-
ARG41323 anti-RSPO1 / R spondin 1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human spleen tissue stained with ARG41323 anti-RSPO1 / R spondin 1 antibody at 2.5 µg/ml dilution.
-
ARG41323 anti-RSPO1 / R spondin 1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human spleen tissue stained with ARG41323 anti-RSPO1 / R spondin 1 antibody at 5 µg/ml dilution.
-
ARG41323 anti-RSPO1 / R spondin 1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human spleen tissue stained with ARG41323 anti-RSPO1 / R spondin 1 antibody (green) at 5 µg/ml dilution. DAPI (blue) for nuclear staining. Phylloidin staining (red).
-
ARG41323 anti-RSPO1 / R spondin 1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human heart tissue stained with ARG41323 anti-RSPO1 / R spondin 1 antibody at 2.5 µg/ml dilution.
