ARG43327
anti-RSPO1 / R spondin 1 antibody
anti-RSPO1 / R spondin 1 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes RSPO1 / R spondin 1 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | RSPO1 / R spondin 1 |
| 抗原物种 | Human |
| 抗原 | Recombinant fusion protein corresponding to aa. 21-263 of Human RSPO1 / R spondin 1 (NP_001033722.1). |
| 偶联标记 | Un-conjugated |
| 別名 | hRspo1; Roof plate-specific spondin-1; R-spondin-1; RSPO; CRISTIN3 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | RSPO1 |
| 全名 | R-spondin 1 |
| 背景介绍 | This gene encodes a secreted activator protein with two cysteine-rich, furin-like domains and one thrombospondin type 1 domain. The encoded protein is a ligand for leucine-rich repeat-containing G-protein coupled receptors (LGR proteins) and positively regulates the Wnt signaling pathway. In mice, the protein induces the rapid onset of crypt cell proliferation and increases intestinal epithelial healing, providing a protective effect against chemotherapy-induced adverse effects. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2014] |
| 生物功能 | Activator of the canonical Wnt signaling pathway by acting as a ligand for LGR4-6 receptors (PubMed:29769720). Upon binding to LGR4-6 (LGR4, LGR5 or LGR6), LGR4-6 associate with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. Also regulates the canonical Wnt/beta-catenin-dependent pathway and non-canonical Wnt signaling by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. Acts as a ligand for frizzled FZD8 and LRP6. May negatively regulate the TGF-beta pathway. Has a essential roles in ovary determination. Regulates Wnt signaling by antagonizing DKK1/KREM1-mediated internalization of LRP6 through an interaction with KREM1 (PubMed:17804805). [UniProt] |
| 细胞定位 | Secreted. Nucleus. Note=Seems to mainly localize to nucleoli. [UniProt] |
| 预测分子量 | 29 kDa |
检测图片 (2) Click the Picture to Zoom In
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ARG43327 anti-RSPO1 / R spondin 1 antibody ICC/IF image
Immunofluorescence: NIH/3T3 cells stained with ARG43327 anti-RSPO1 / R spondin 1 antibody at 1:100 dilution.
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ARG43327 anti-RSPO1 / R spondin 1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat ovary tissue stained with ARG43327 anti-RSPO1 / R spondin 1 antibody at 1:100 dilution.
