ARG22271
anti-Rhodopsin antibody [4D2]
anti-Rhodopsin antibody [4D2] for ELISA,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Rat,Amphibians,Bird,Fish,Mammal,Shark
概述
| 产品描述 | Mouse Monoclonal antibody [4D2] recognizes Rhodopsin |
|---|---|
| 反应物种 | Hu, Ms, Rat, Amph, Bird, Fsh, Mamm, Shark |
| 不反应物种 | Invt |
| 应用 | ELISA, ICC/IF, IHC-P, IP, WB |
| 特异性 | Detects ~40kDa. Binds specifically to the N-terminus of Rhodopsin. Does not detect Rhodopsin in invertebrates. |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 4D2 |
| 同位型 | IgG1 |
| 靶点名称 | Rhodopsin |
| 抗原物种 | Bovine |
| 抗原 | Bovine Rhodopsin |
| 偶联标记 | Un-conjugated |
| 別名 | Rhodopsin; Opsin-2; CSNBAD1; RP4; OPN2 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 缓冲液 | PBS (pH 7.4), 0.09% Sodium azide and 50% Glycerol |
| 抗菌剂 | 0.09% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | RHO |
| 全名 | rhodopsin |
| 背景介绍 | Retinitis pigmentosa is an inherited progressive disease which is a major cause of blindness in western communities. It can be inherited as an autosomal dominant, autosomal recessive, or X-linked recessive disorder. In the autosomal dominant form,which comprises about 25% of total cases, approximately 30% of families have mutations in the gene encoding the rod photoreceptor-specific protein rhodopsin. This is the transmembrane protein which, when photoexcited, initiates the visual transduction cascade. Defects in this gene are also one of the causes of congenital stationary night blindness. [provided by RefSeq, Jul 2008] |
| 生物功能 | Photoreceptor required for image-forming vision at low light intensity. Required for photoreceptor cell viability after birth. Light-induced isomerization of 11-cis to all-trans retinal triggers a conformational change leading to G-protein activation and release of all-trans retinal. [UniProt] |
| 细胞定位 | Membrane |
| 预测分子量 | 39 kDa |
| 翻译后修饰 | Phosphorylated on some or all of the serine and threonine residues present in the C-terminal region. Contains one covalently linked retinal chromophore. |
检测图片 (1) Click the Picture to Zoom In
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ARG22271 anti-Rhodopsin antibody [4D2] IHC image
Immunohistochemistry: Mouse retina stained with ARG22271 anti-Rhodopsin antibody [4D2] at 1:1000 dilution. Other layers of the retina: IS - inner segment; ONL - outer nuclear layer; OPL - outer plexiform layer; INL - inner nuclear layer; IPL - inner plexiform layer; GCL - ganglion cell layer.
