ARG41965

anti-S100A4 antibody [S100A4/1482]

anti-S100A4 antibody [S100A4/1482] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse

概述

产品描述 Mouse Monoclonal antibody [S100A4/1482] recognizes S100A4
反应物种 Hu, Ms
应用 FACS, ICC/IF, IHC-P, WB
宿主 Mouse
克隆 Monoclonal
克隆号 S100A4/1482
同位型 IgG1, kappa
靶点名称 S100A4
抗原物种 Human
抗原 Recombinant protein of Human S100A4.
偶联标记 Un-conjugated
別名 S100 calcium-binding protein A4; Placental calcium-binding protein; 42A; MTS1; FSP1; CAPL; Metastasin; Protein S100-A4; P9KA; 18A2; Protein Mts1; Calvasculin; PEL98

应用说明

应用建议
应用 推荐稀释比
FACS1 - 2 µg/10^6 cells
ICC/IF2 - 5 µg/ml
IHC-P2 - 5 µg/ml
WB1 - 2 µg/ml
应用说明 IHC-P: Antigen Retrieval: Steam tissue section in 10 mM Citrate buffer (pH 6.0) for 10-20 min, followed by cooling.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
阳性对照 HeLa
实际分子量 ~ 12 kDa

属性

形式 Liquid
纯化 Purification with Protein G.
缓冲液 PBS, 0.05% Sodium azide and 0.1 mg/ml BSA.
抗菌剂 0.05% Sodium azide
稳定剂 0.1 mg/ml BSA
浓度 0.2 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 20198 Mouse S100A4

GeneID: 6275 Human S100A4

Swiss-port # P07091 Mouse Protein S100-A4

Swiss-port # P26447 Human Protein S100-A4

基因名称 S100A4
全名 S100 calcium binding protein A4
背景介绍 The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in motility, invasion, and tubulin polymerization. Chromosomal rearrangements and altered expression of this gene have been implicated in tumor metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008]
产品亮点 Related products:
S100A4 antibodies; S100A4 Duos / Panels; Anti-Mouse IgG secondary antibodies;
Related news:
New antibody panels for Myofibroblasts and CAFs
预测分子量 12 kDa

检测图片 (6) Click the Picture to Zoom In

  • ARG41965 anti-S100A4 antibody [S100A4/1482] ICC/IF image

    Immunofluorescence: Fixed and permeabilized HeLa cells stained with ARG41965 anti-S100A4 antibody [S100A4/1482] (green). Reddot (red) for nuclear staining.

  • ARG41965 anti-S100A4 antibody [S100A4/1482] IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded Human placenta tissue. Antigen Retrieval: Steam tissue section in 10 mM Citrate buffer (pH 6.0) for 10-20 min followed by cooling. The tissue section was stained with ARG41965 anti-S100A4 antibody [S100A4/1482].

  • ARG41965 anti-S100A4 antibody [S100A4/1482] WB image

    Western blot: HeLa, A549 and A431 cell lysates stained with ARG41965 anti-S100A4 antibody [S100A4/1482].

  • ARG41965 anti-S100A4 antibody [S100A4/1482] WB image

    Western blot: HeLa cell lysate stained with ARG41965 anti-S100A4 antibody [S100A4/1482].

  • ARG41965 anti-S100A4 antibody [S100A4/1482] FACS image

    Flow Cytometry: Human T98G cells stained with ARG41965 anti-S100A4 antibody [S100A4/1482] (blue) or isotype control (red).

  • ARG41965 anti-S100A4 antibody [S100A4/1482] FACS image

    Flow Cytometry: Human A549 cells stained with ARG41965 anti-S100A4 antibody [S100A4/1482] (blue) or isotype control (red).