ARG11154
anti-SARS-CoV / SARS-CoV-2 Spike protein (RBD) antibody
anti-SARS-CoV / SARS-CoV-2 Spike protein (RBD) antibody for ICC/IF,Western blot and Virus
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes SARS-CoV / SARS-CoV-2 Spike protein (RBD) |
|---|---|
| 反应物种 | Virus |
| 应用 | ICC/IF, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | SARS-CoV / SARS-CoV-2 Spike protein (RBD) |
| 抗原物种 | Virus |
| 抗原 | Recombinant SARS-CoV-2 Spike protein ACE2 binding domain (aa. 308-541 in the Spike protein sequence in Wuhan-Hu-1). |
| 偶联标记 | Un-conjugated |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified |
| 缓冲液 | PBS, 5 mM Sodium azide and 50% Glycerol. |
| 抗菌剂 | 5 mM Sodium azide |
| 稳定剂 | 50% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
检测图片 (4) Click the Picture to Zoom In
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ARG11154 anti-SARS-CoV / SARS-CoV-2 Spike protein (RBD) antibody ICC/IF image
Immunofluorescence: HEK293 cells transfected with pCI-Neo-Mod vector including DNA encoding the SARS-CoV-2 Spike protein ACE2 binding domain (aa. 308-541 in the Spike protein sequence in Wuhan-Hu-1). This region was used as the immunogen for development of ARG11154 anti-SARS-CoV / SARS-CoV-2 Spike protein (RBD) antibody. HEK293 cells stained with ARG11154 (red) at 1:3000 dilution, and co-stained with anti-beta Tubulin antibody (green) at 1:5000 dilution. Hoechst (blue) for nuclear staining.
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ARG11154 anti-SARS-CoV / SARS-CoV-2 Spike protein (RBD) antibody WB image
Western blot: 1) Non-transfected HEK293 cells, 2) HEK293 cells transfected with pCI-Neo-Mod vector containing the SARS-CoV-2 Spike protein ACE2 binding domain cDNA, and 3) HEK293 cells transfected with pCI-Neo-GFP vector containing the SARS-CoV-2 Spike protein ACE2 binding domain cDNA. Cell lysates were stained with ARG11154 anti-SARS-CoV / SARS-CoV-2 Spike protein (RBD) antibody (green) at 1:2000 dilution. The same blot was simultaneously stained with anti-beta Tubulin antibody (red) at 1:5000 dilution.
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ARG11154 anti-SARS-CoV / SARS-CoV-2 Spike protein (RBD) antibody ICC/IF image
Immunofluorescence: Vero cells infected with SARS-CoV-2 virus. Cells were stained with ARG11154 anti-SARS-CoV / SARS-CoV-2 Spike protein (RBD) antibody (red). DAPI (blue) for nuclear staining.
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ARG11154 anti-SARS-CoV / SARS-CoV-2 Spike protein (RBD) antibody WB image
Western blot: Full-length recombinant SARS-CoV-2 Spike protein expressed in HEK293 cells. Loading of 1 µg (left) or 100 ng (right). The blots were stained with ARG11154 anti-SARS-CoV / SARS-CoV-2 Spike protein (RBD) antibody.
