ARG51672
anti-SEK1 / MKK4 phospho (Thr261) antibody
anti-SEK1 / MKK4 phospho (Thr261) antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections and Human,Mouse,Rat
Signaling Transduction antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes SEK1 / MKK4 phospho (Thr261) |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ICC/IF, IHC-P |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | SEK1 / MKK4 |
| 抗原物种 | Human |
| 抗原 | Peptide sequence around phosphorylation site of threonine261 (A-K-T(p)-RD) derived from Human SEK1/MKK4. |
| 偶联标记 | Un-conjugated |
| 別名 | MEK 4; MAPK/ERK kinase 4; PRKMK4; SAPKK-1; SAPK/ERK kinase 1; SKK1; JNK-activating kinase 1; EC 2.7.12.2; MEK4; MAP kinase kinase 4; c-Jun N-terminal kinase kinase 1; SEK1; SAPKK1; MAPKK4; Stress-activated protein kinase kinase 1; JNKK1; MKK4; SERK1; SAPK kinase 1; Dual specificity mitogen-activated protein kinase kinase 4; JNKK; MAPKK 4 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic phosphopeptide. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. In addition, non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| 缓冲液 | PBS (without Mg2+ and Ca2+, pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P45985 Human Dual specificity mitogen-activated protein kinase kinase 4 Swiss-port # P47809 Mouse Dual specificity mitogen-activated protein kinase kinase 4 |
|---|---|
| 基因名称 | MAP2K4 |
| 全名 | mitogen-activated protein kinase kinase 4 |
| 背景介绍 | Dual specificity kinase that activates the JUN kinases MAPK8 (JNK1) and MAPK9 (JNK2) as well as MAPK14 (p38) but not MAPK1 (ERK2) or MAPK3 (ERK1). |
| 生物功能 | Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Essential component of the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. With MAP2K7/MKK7, is the one of the only known kinase to directly activate the stress-activated protein kinase/c-Jun N-terminal kinases MAPK8/JNK1, MAPK9/JNK2 and MAPK10/JNK3. MAP2K4/MKK4 and MAP2K7/MKK7 both activate the JNKs by phosphorylation, but they differ in their preference for the phosphorylation site in the Thr-Pro-Tyr motif. MAP2K4 shows preference for phosphorylation of the Tyr residue and MAP2K7/MKK7 for the Thr residue. The phosphorylation of the Thr residue by MAP2K7/MKK7 seems to be the prerequisite for JNK activation at least in response to proinflammatory cytokines, while other stimuli activate both MAP2K4/MKK4 and MAP2K7/MKK7 which synergistically phosphorylate JNKs. MAP2K4 is required for maintaining peripheral lymphoid homeostasis. The MKK/JNK signaling pathway is also involved in mitochondrial death signaling pathway, including the release cytochrome c, leading to apoptosis. Whereas MAP2K7/MKK7 exclusively activates JNKs, MAP2K4/MKK4 additionally activates the p38 MAPKs MAPK11, MAPK12, MAPK13 and MAPK14. [UniProt] |
| 研究领域 | Signaling Transduction antibody |
| 预测分子量 | 44 kDa |
| 翻译后修饰 | Activated by phosphorylation on Ser-257 and Thr-261 by MAP kinase kinase kinases (MAP3Ks). |
检测图片 (2) Click the Picture to Zoom In
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ARG51672 anti-SEK1 / MKK4 phospho (Thr261) antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human breast carcinoma tissue stained with ARG51672 anti-SEK1 / MKK4 phospho (Thr261) antibody (left) or the same antibody preincubated with blocking peptide (right).
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ARG51672 anti-SEK1 / MKK4 phospho (Thr261) antibody ICC/IF image
Immunofluorescence: methanol-fixed HeLa cells stained with ARG51672 anti-SEK1 / MKK4 phospho (Thr261) antibody.
