ARG59546
anti-SFTPA1 antibody
anti-SFTPA1 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes SFTPA1 |
|---|---|
| 反应物种 | Ms, Rat |
| 预测物种 | Hm |
| 应用 | IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | SFTPA1 |
| 抗原物种 | Mouse |
| 抗原 | Synthetic peptide corresponding to aa. 233-248 of Mouse SFTPA1. (WNDKGCLQYRLAICEF) |
| 偶联标记 | Un-conjugated |
| 別名 | SFTPA1B; SP-A; COLEC4; Pulmonary surfactant-associated protein A1; PSAP; PSPA; 35 kDa pulmonary surfactant-associated protein; SFTP1; PSP-A; SPA; SPA1; Alveolar proteinosis protein; SP-A1; Collectin-4 |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|---|---|
| 应用说明 | IHC-P: Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | 0.9% NaCl, 0.2% Na2HPO4, 0.05% Thimerosal, 0.05% Sodium azide and 5% BSA. |
| 抗菌剂 | 0.05% Thimerosal and 0.05% Sodium azide |
| 稳定剂 | 5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P08427 Rat Pulmonary surfactant-associated protein A |
|---|---|
| 基因名称 | SFTPA1 |
| 全名 | surfactant protein A1 |
| 背景介绍 | This gene encodes a lung surfactant protein that is a member of a subfamily of C-type lectins called collectins. The encoded protein binds specific carbohydrate moieties found on lipids and on the surface of microorganisms. This protein plays an essential role in surfactant homeostasis and in the defense against respiratory pathogens. Mutations in this gene are associated with idiopathic pulmonary fibrosis. Alternate splicing results in multiple transcript variants. [provided by RefSeq, May 2010] |
| 生物功能 | In presence of calcium ions, it binds to surfactant phospholipids and contributes to lower the surface tension at the air-liquid interface in the alveoli of the mammalian lung and is essential for normal respiration. [UniProt] |
| 细胞定位 | Secreted, extracellular space, extracellular matrix. Secreted, extracellular space, surface film. [UniProt] |
| 预测分子量 | 26 kDa |
| 翻译后修饰 | N-acetylated. [UniProt] |
检测图片 (3) Click the Picture to Zoom In
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ARG59546 anti-SFTPA1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat lung tissues. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG59546 anti-SFTPA1 antibody at 1 µg/ml, overnight at 4°C.
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ARG59546 anti-SFTPA1 antibody WB image
Western blot: 50 µg of samples under reducing conditions. Mouse lung lysates (Lane 1, 2) and Rat lung lysates (Lane 3, 4) stained with ARG59546 anti-SFTPA1 antibody at 0.5 µg/ml, overnight at 4°C.
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ARG59546 anti-SFTPA1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse lung tissues. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG59546 anti-SFTPA1 antibody at 1 µg/ml, overnight at 4°C.
