ARG65697

Name: anti-SIRT1 antibody
Brand: arigo Biolaboratories
SKU: ARG65697
Price: 275 USD
Availability: InStock
Rating: 5.0 (1 reviews)

anti-SIRT1 antibody

Datasheet

订购信息

包装

价格 (USD)

50 μl

275

arigo in CHINA:
恩瑞科生物
☎ 400-886-0853,
0571-8708-1890

概述

产品描述	Rabbit Polyclonal antibody recognizes SIRT1
反应物种	Hu, Ms
应用	FACS, IHC-P, WB
宿主	Rabbit
克隆	Polyclonal
同位型	IgG
靶点名称	SIRT1
抗原物种	Human
抗原	KLH-conjugated synthetic peptide around the C-terminus of Human SIRT1.
偶联标记	Un-conjugated
別名	75SirT1; SIR2L1; SIR2alpha; SIR2-like protein 1; EC 3.5.1.-; NAD-dependent protein deacetylase sirtuin-1; SIR2; hSIRT1; Regulatory protein SIR2 homolog 1; hSIR2

应用说明

应用建议

应用	推荐稀释比
FACS	1:100 - 1:200
IHC-P	1:200
WB	1:1000 - 1:2000

应用说明

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

阳性对照

Hela, F9, Jurkat, human lung carcinoma tissue, human colon carcinoma tissue

属性

形式	Liquid
纯化	Affinity purification with immunogen.
缓冲液	1*TBS (pH 7.4), 0.05% Sodium azide, 0.5% BSA and 40% Glycerol
抗菌剂	0.05% Sodium azide
稳定剂	0.5% BSA, 40% Glycerol
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 23411 Human SIRT1 GeneID: 93759 Mouse SIRT1 Swiss-port # Q923E4 Mouse NAD-dependent protein deacetylase sirtuin-1 Swiss-port # Q96EB6 Human NAD-dependent protein deacetylase sirtuin-1
基因名称	SIRT1
全名	sirtuin 1
背景介绍	This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2008]
研究领域	Cell Biology and Cellular Response antibody; Cell Death antibody; Controls and Markers antibody; Gene Regulation antibody; Metabolism antibody; Microbiology and Infectious Disease antibody
预测分子量	82 kDa
翻译后修饰	Methylated on multiple lysine residues; methylation is enhanced after DNA damage and is dispensable for deacetylase activity toward p53/TP53. Phosphorylated. Phosphorylated by STK4/MST1, resulting in inhibition of SIRT1-mediated p53/TP53 deacetylation. Phosphorylation by MAPK8/JNK1 at Ser-27, Ser-47, and Thr-530 leads to increased nuclear localization and enzymatic activity. Phosphorylation at Thr-530 by DYRK1A and DYRK3 activates deacetylase activity and promotes cell survival. Phosphorylation by mammalian target of rapamycin complex 1 (mTORC1) at Ser-47 inhibits deacetylation activity. Phosphorylated by CaMK2, leading to increased p53/TP53 and NF-kappa-B p65/RELA deacetylation activity (By similarity). Phosphorylation at Ser-27 implicating MAPK9 is linked to protein stability. There is some ambiguity for some phosphosites: Ser-159/Ser-162 and Thr-544/Ser-545. Proteolytically cleaved by cathepsin B upon TNF-alpha treatment to yield catalytic inactive but stable SirtT1 75 kDa fragment (75SirT1). S-nitrosylated by GAPDH, leading to inhibit the NAD-dependent protein deacetylase activity.

检测图片 (2) Click the Picture to Zoom In

ARG65697 anti-SIRT1 antibody WB image

Western blot: 30 µg of HCT116 cell lysate stained with ARG65697 anti-SIRT1 antibody at 1:1000 dilution.
ARG65697 anti-SIRT1 antibody WB image

Western blot: 30 µg of HeLa cell lysate stained with ARG65697 anti-SIRT1 antibody at 1:1000 dilution.