ARG59530
anti-SMPD1 / Acid Sphingomyelinase antibody
anti-SMPD1 / Acid Sphingomyelinase antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes SMPD1 / Acid Sphingomyelinase |
|---|---|
| 反应物种 | Hu |
| 预测物种 | Bov |
| 应用 | FACS, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | SMPD1 / Acid Sphingomyelinase |
| 抗原物种 | Human |
| 抗原 | KLH-conjugated synthetic peptide corresponding to aa. 391-419 of Human SMPD1 / Acid Sphingomyelinase. |
| 偶联标记 | Un-conjugated |
| 別名 | aSMase; EC 3.1.4.12; NPD; ASMASE; Sphingomyelin phosphodiesterase; Acid sphingomyelinase; ASM |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 阳性对照 | Human cerebellum |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A and immunogen peptide. |
| 缓冲液 | PBS and 0.09% (W/V) Sodium azide. |
| 抗菌剂 | 0.09% (W/V) Sodium azide. |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | SMPD1 |
| 全名 | sphingomyelin phosphodiesterase 1, acid lysosomal |
| 背景介绍 | The protein encoded by this gene is a lysosomal acid sphingomyelinase that converts sphingomyelin to ceramide. The encoded protein also has phospholipase C activity. Defects in this gene are a cause of Niemann-Pick disease type A (NPA) and Niemann-Pick disease type B (NPB). Multiple transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2010] |
| 生物功能 | Converts sphingomyelin to ceramide. Also has phospholipase C activities toward 1,2-diacylglycerolphosphocholine and 1,2-diacylglycerolphosphoglycerol. Isoform 2 and isoform 3 have lost catalytic activity. [UniProt] |
| 细胞定位 | Lysosome. Secreted. [UniProt] |
| 预测分子量 | 70 kDa |
检测图片 (3) Click the Picture to Zoom In
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ARG59530 anti-SMPD1 / Acid Sphingomyelinase antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human testis stained with ARG59530 anti-SMPD1 / Acid Sphingomyelinase antibody.
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ARG59530 anti-SMPD1 / Acid Sphingomyelinase antibody WB image
Western blot: 20 µg of Human cerebellum lysate stained with ARG59530 anti-SMPD1 / Acid Sphingomyelinase antibody at 1:2000 dilution.
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ARG59530 anti-SMPD1 / Acid Sphingomyelinase antibody FACS image
Flow Cytometry: K562 cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then incubated in 2% BSA to block non-specific protein-protein interactions and stained with ARG59530 anti-SMPD1 / Acid Sphingomyelinase antibody (right histogram) at 1:25 dilution for 60 min at 37°C, followed by DyLight®488 labelled secondary antibody. Isotype control antibody (left histogram) was Rabbit IgG (1 µg/10^6 cells) used under the same conditions. Acquisition of > 10000 events was performed.
