ARG54002
anti-SOX2 antibody
anti-SOX2 antibody for Flow cytometry,Western blot and Human,Mouse
Developmental Biology antibody; Gene Regulation antibody; Neuroscience antibody
概述
| 产品描述 | Mouse Monoclonal antibody recognizes SOX2 |
|---|---|
| 反应物种 | Hu, Ms |
| 应用 | FACS, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 同位型 | IgG1 |
| 靶点名称 | SOX2 |
| 抗原物种 | Mouse |
| 抗原 | Purified recombinant mouse Sox2 protein fragments expressed in E.coli |
| 偶联标记 | Un-conjugated |
| 別名 | Transcription factor SOX-2; MCOPS3; ANOP3 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 实际分子量 | 35 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified |
| 缓冲液 | 0.1M Tris-Glycine (pH 7.4), 150 mM NaCl, 0.2% Sodium azide and 50% Glycerol |
| 抗菌剂 | 0.2% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | Sox2 |
| 全名 | SRY (sex determining region Y)-box 2 |
| 生物功能 | Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency. May function as a switch in neuronal development. Downstream SRRT target that mediates the promotion of neural stem cell self-renewal. Keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation (By similarity). [UniProt] |
| 细胞定位 | Nucleus |
| 研究领域 | Developmental Biology antibody; Gene Regulation antibody; Neuroscience antibody |
| 预测分子量 | 34 kDa |
| 翻译后修饰 | Sumoylation inhibits binding on DNA and negatively regulates the FGF4 transactivation. |
检测图片 (3) Click the Picture to Zoom In
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ARG54002 anti-SOX2 antibody WB image
Western blot: 100 µg of whole cell lysates from shSOX2 #1, shSOX2 #2 or control transfected 5637 cells (Human bladder cancer cell line) stained with ARG54002 anti-SOX2 antibody at 1:1000 dilution.
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ARG54002 anti-Sox2 antibody FACS image
Flow Cytometry: F9 cells stained with ARG54002 anti-Sox2 antibody at 1:100 dilution (green), followed by FITC-conjugated goat mouse IgG. Isotype control, normal mouse IgG (black).
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ARG54002 anti-Sox2 antibody WB image
Western blot: Mouse F9 cell lysates stained with ARG54002 anti-Sox2 antibody at 1:1000 dilution.
客户反馈
Excellent
anti-SOX2 antibody
Application：WB
Sample：5637 (Human bladder cancer cell line), knockdown SOX2.
Sample Loading Amount：100 µg / whole cell lysate
Primary Antibody Dilution Factor：1:1000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
