ARG57700
anti-SPARC antibody
anti-SPARC antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes SPARC |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | SPARC |
| 抗原物种 | Human |
| 抗原 | Recombinant protein of Human SPARC. |
| 偶联标记 | Un-conjugated |
| 別名 | ON; BM-40; Basement-membrane protein 40; SPARC; Osteonectin; Secreted protein acidic and rich in cysteine |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 阳性对照 | A375 |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | SPARC |
| 全名 | secreted protein, acidic, cysteine-rich (osteonectin) |
| 背景介绍 | This gene encodes a cysteine-rich acidic matrix-associated protein. The encoded protein is required for the collagen in bone to become calcified but is also involved in extracellular matrix synthesis and promotion of changes to cell shape. The gene product has been associated with tumor suppression but has also been correlated with metastasis based on changes to cell shape which can promote tumor cell invasion. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2015] |
| 生物功能 | Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity. [UniProt] |
| 细胞定位 | Secreted, basement membrane, extracellular matrix, extracellular space. [UniProt] |
| 预测分子量 | 35 kDa |
检测图片 (2) Click the Picture to Zoom In
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ARG57700 anti-SPARC antibody ICC/IF image
Immunofluorescence: U2OS cells stained with ARG57700 anti-SPARC antibody at 1:100 dilution.
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ARG57700 anti-SPARC antibody WB image
Western blot: 25 µg of A375 cell lysate stained with ARG57700 anti-SPARC antibody at 1:3000 dilution.
