ARG51823
anti-STAT2 phospho (Tyr690) antibody
anti-STAT2 phospho (Tyr690) antibody for Western blot and Human
Gene Regulation antibody; Signaling Transduction antibody
参考文献1
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes STAT2 phospho (Tyr690) |
|---|---|
| 反应物种 | Hu |
| 应用 | WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | STAT2 |
| 抗原物种 | Human |
| 抗原 | Peptide sequence around phosphorylation site of tyrosine 690 (R-K-Y(p)-L-K)derived from Human Stat2 |
| 偶联标记 | Un-conjugated |
| 別名 | P113; Signal transducer and activator of transcription 2; STAT113; p113; ISGF-3 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic phosphopeptide. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. In addition, non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| 缓冲液 | PBS (without Mg2+ and Ca2+, pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P52630 Human Signal transducer and activator of transcription 2 |
|---|---|
| 基因名称 | STAT2 |
| 全名 | signal transducer and activator of transcription 2, 113kDa |
| 背景介绍 | The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. In response to interferon (IFN), this protein forms a complex with STAT1 and IFN regulatory factor family protein p48 (ISGF3G), in which this protein acts as a transactivator, but lacks the ability to bind DNA directly. Transcription adaptor P300/CBP (EP300/CREBBP) has been shown to interact specifically with this protein, which is thought to be involved in the process of blocking IFN-alpha response by adenovirus. Multiple transcript variants encoding different isoforms have been found for this gene. |
| 生物功能 | Signal transducer and activator of transcription that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with IRF9/ISGF3G to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state. [UniProt] |
| 产品亮点 | Related products: STAT2 antibodies; Anti-Rabbit IgG secondary antibodies; Related news: Exploring Antiviral Immune Response circNDUFB2, a circular RNA (circRNA), activates anti-tumor immunity |
| 研究领域 | Gene Regulation antibody; Signaling Transduction antibody |
| 预测分子量 | 98 kDa |
| 翻译后修饰 | Tyrosine phosphorylated in response to IFN-alpha. Phosphorylation at Ser-287 negatively regulates the transcriptional response. |
检测图片 (1) Click the Picture to Zoom In
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ARG51823 anti-STAT2 phospho (Tyr690) antibody WB image
Western blot: Extracts from HeLa cells untreated or treated with IFN-a stained with ARG51823 anti-STAT2 phospho (Tyr690) antibody.
文献引用
The Different Effects of IFN- β and IFN- γ on the Tumor-Suppressive Activity of Human Amniotic Fluid-Derived Mesenchymal Stem Cells
WB / Human
