ARG55429
anti-STUB1 / CHIP antibody
anti-STUB1 / CHIP antibody for Flow cytometry,Western blot and Human
Gene Regulation antibody; Signaling Transduction antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes STUB1 / CHIP |
|---|---|
| 反应物种 | Hu |
| 预测物种 | Ms, Chk |
| 应用 | FACS, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | STUB1 / CHIP |
| 抗原物种 | Human |
| 抗原 | KLH-conjugated synthetic peptide corresponding to aa. 236-263 (C-terminus) of Human STUB1. |
| 偶联标记 | Un-conjugated |
| 別名 | NY-CO-7; Antigen NY-CO-7; UBOX1; EC 6.3.2.-; E3 ubiquitin-protein ligase CHIP; SDCCAG7; CHIP; STIP1 homology and U box-containing protein 1; HSPABP2; SCAR16; Carboxy terminus of Hsp70-interacting protein; CLL-associated antigen KW-8 |
应用说明
| 应用建议 |
|
||||||
|---|---|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | 293 |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A and immunogen peptide. |
| 缓冲液 | PBS and 0.09% (W/V) Sodium azide |
| 抗菌剂 | 0.09% (W/V) Sodium azide |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | STUB1 |
| 全名 | STIP1 homology and U-box containing protein 1, E3 ubiquitin protein ligase |
| 背景介绍 | This gene encodes a protein containing tetratricopeptide repeat and a U-box that functions as a ubiquitin ligase/cochaperone. The encoded protein binds to and ubiquitinates shock cognate 71 kDa protein (Hspa8) and DNA polymerase beta (Polb), among other targets. Mutations in this gene cause spinocerebellar ataxia, autosomal recessive 16. Alternative splicing results in multiple transcript variants. There is a pseudogene for this gene on chromosome 2. [provided by RefSeq, Jun 2014] |
| 生物功能 | E3 ubiquitin-protein ligase which targets misfolded chaperone substrates towards proteasomal degradation. Collaborates with ATXN3 in the degradation of misfolded chaperone substrates: ATXN3 restricting the length of ubiquitin chain attached to STUB1/CHIP substrates and preventing further chain extension. Ubiquitinates NOS1 in concert with Hsp70 and Hsp40. Modulates the activity of several chaperone complexes, including Hsp70, Hsc70 and Hsp90. Mediates transfer of non-canonical short ubiquitin chains to HSPA8 that have no effect on HSPA8 degradation. Mediates polyubiquitination of DNA polymerase beta (POLB) at 'Lys-41', 'Lys-61' and 'Lys-81', thereby playing a role in base-excision repair: catalyzes polyubiquitination by amplifying the HUWE1/ARF-BP1-dependent monoubiquitination and leading to POLB-degradation by the proteasome. Mediates polyubiquitination of CYP3A4. Ubiquitinates EPHA2 and may regulate the receptor stability and activity through proteasomal degradation. Negatively regulates the suppressive function of regulatory T-cells (Treg) during inflammation by mediating the ubiquitination and degradation of FOXP3 in a HSPA1A/B-dependent manner. [UniProt] |
| 细胞定位 | Cytoplasm. |
| 研究领域 | Gene Regulation antibody; Signaling Transduction antibody |
| 预测分子量 | 35 kDa |
| 翻译后修饰 | Monoubiquitinated at Lys-2 following cell stress by UBE2W, promoting the interaction with ATXN3 (By similarity). Auto-ubiquitinated; mediated by UBE2D1 and UBE2D2. |
检测图片 (2) Click the Picture to Zoom In
-
ARG55429 anti-STUB1 / CHIP antibody WB image
Western blot: 35 µg of 293 cell lysate stained with ARG55429 anti-STUB1 / CHIP antibody.
-
ARG55429 anti-STUB1 / CHIP antibody FACS image
Flow Cytometry: K562 cells stained with ARG55429 anti-STUB1 / CHIP antibody (right histogram) or without primary antibody control (left histogram), followed by incubation with FITC labelled secondary antibody.
