ARG52422
anti-Serotonin Transporter phospho (Thr 276) antibody
anti-Serotonin Transporter phospho (Thr 276) antibody for Western blot and Rat
Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes Serotonin Transporter phospho (Thr 276) |
|---|---|
| 反应物种 | Rat |
| 预测物种 | Hu, Ms, Bov, NHuPrm, Sheep, Xenopus laevis |
| 应用 | WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | Serotonin Transporter |
| 抗原物种 | Rat |
| 抗原 | Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr276 conjugated to KLH |
| 偶联标记 | Un-conjugated |
| 別名 | SERT; 5HTT; SERT1; Sodium-dependent serotonin transporter; hSERT; 5-HTTLPR; Solute carrier family 6 member 4; HTT; 5-HTT; 5HT transporter; OCD1 |
应用说明
| 应用建议 |
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| 应用说明 | Specific for the ~76k SERT protein phosphorylated at Ser 276. Immunolabeling is blocked by preadsorption of antibody with the phospho-peptide that was used to geneRate the antibody but not by the corresponding dephospho-peptide. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity Purified |
| 缓冲液 | 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% Glycerol |
| 稳定剂 | 0.1 mg/ml BSA, 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P31652 Rat Sodium-dependent serotonin transporter |
|---|---|
| 基因名称 | Slc6a4 |
| 全名 | solute carrier family 6 (neurotransmitter transporter), member 4 |
| 背景介绍 | The serotonin transporter (SERT) recycles serotonin by transporting it back to the pre-synaptic cell. It is the primary target for most anti-depressant drugs and for stimulants such as methamphetamines. SERT is regulated by several processes, including a cyclic GMP signaling pathway involving nitric oxide synthase, guanylyl cyclase, and cGMP-dependent protein kinase (PKG). cGMP- and PKG-mediated SERT regulation requires phosphorylation at thr276 (Ramamoorthy et al., 2007). It has been suggested that although PKG is involved in the stimulation of SERT at thr276, it does not directly phosphorylate the residue, rather it initiates a kinase cascade that leads to SERT phosphorylation by an as yet unidentified protein kinase (Wong et al., 2012). Also of therapeutic importance, mutation at the thr276 residue has been shown to decrease the potency of a variety of anti-depressant drugs, (Zhang YW and Rudnick G, 2005). |
| 研究领域 | Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody |
| 预测分子量 | 70 kDa |
| 翻译后修饰 | Glycosylated; modification with sialylated N-glycans is a requirement for transporters to associate with each other and to function as homooligomeric forms. Phosphorylation at Thr-276 increases 5-HT uptake and is required for cGMP-mediated SERT regulation. Phosphorylation upon PKC stimulation modifies the SERT distribution and density in the membrane, and diminishes the uptake capacity. |
检测图片 (1) Click the Picture to Zoom In
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ARG52422 anti-Serotonin Transporter phospho (Thr 276) antibody WB image
Western blot: Rat mid brain membrane lysate showing specific immunolabeling of the ~76k SERT protein phosphorylated at Thr 276 ARG52422 anti-Serotonin Transporter phospho (Thr 276) antibody.
Immunolabeling is blocked by preadsorption with the peptide used as antigen.
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