ARG55400
anti-TET3 antibody
anti-TET3 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Gene Regulation antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes TET3 |
|---|---|
| 反应物种 | Hu |
| 应用 | IHC-P, WB |
| 特异性 | At least three isoforms of TET3 are known to exist. This anti-TET3 antibody is predicted to not cross react with other TET proteins. |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | TET3 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide (17 aa) within aa. 1520-1570 of Human TET3. |
| 偶联标记 | Un-conjugated |
| 別名 | Methylcytosine dioxygenase TET3; B430006D22Rik; BC037432; D230004J03Rik; EC 1.14.11.n2 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | SK-N-SH Cell Lysate |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | PBS and 0.02% Sodium azide |
| 抗菌剂 | 0.02% Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | TET3 |
| 全名 | tet methylcytosine dioxygenase 3 |
| 背景介绍 | Members of the ten-eleven translocation (TET) gene family, including TET3, play a role in the DNA methylation process (Langemeijer et al., 2009 [PubMed 19923888]). [supplied by OMIM, Nov 2010] |
| 生物功能 | Dioxygenase that catalyzes the conversion of the modified genomic base 5-methylcytosine (5mC) into 5-hydroxymethylcytosine (5hmC) and plays a key role in epigenetic chromatin reprogramming in the zygote following fertilization. Also mediates subsequent conversion of 5hmC into 5-formylcytosine (5fC), and conversion of 5fC to 5-carboxylcytosine (5caC). Conversion of 5mC into 5hmC, 5fC and 5caC probably constitutes the first step in cytosine demethylation. In zygotes, DNA demethylation occurs selectively in the paternal pronucleus before the first cell division, while the adjacent maternal pronucleus and certain paternally-imprinted loci are protected from this process. Participates in DNA demethylation in the paternal pronucleus by mediating conversion of 5mC into 5hmC, 5fC and 5caC. Does not mediate DNA demethylation of maternal pronucleus because of the presence of DPPA3/PGC7 on maternal chromatin that prevents TET3-binding to chromatin. In addition to its role in DNA demethylation, also involved in the recruitment of the O-GlcNAc transferase OGT to CpG-rich transcription start sites of active genes, thereby promoting histone H2B GlcNAcylation by OGT. [UniProt] |
| 细胞定位 | Chromosome, Cytoplasm, Nucleus. [UniProt] |
| 研究领域 | Gene Regulation antibody |
| 预测分子量 | 194 kDa |
| 翻译后修饰 | Isopeptide bond, Ubl conjugation. [UniProt] |
检测图片 (2) Click the Picture to Zoom In
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ARG55400 anti-TET3 antibody WB image
Western blot: SK-N-SH cell lysate stained with ARG55400 anti-TET3 antibody at 1 µg/ml dilution in (A) the absence and (B) the presence of blocking peptide.
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ARG55400 anti-TET3 antibody IHC image
Immunohistochemistry: Human brain tissue stained with ARG55400 anti-TET3 antibody at 20 µg/ml dilution.
