ARG20584
anti-TIMP2 antibody [3A4]
anti-TIMP2 antibody [3A4] for IHC-Formalin-fixed paraffin-embedded sections,IHC-Whole mount,Western blot and Human,Zebrafish
概述
| 产品描述 | Mouse Monoclonal antibody [3A4] recognizes TIMP2 |
|---|---|
| 反应物种 | Hu, Zfsh |
| 预测物种 | Ms, Rat, Chk, Pig, Rb |
| 应用 | IHC-P, IHC-Wmt, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 3A4 |
| 同位型 | IgG2a |
| 靶点名称 | TIMP2 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide from Human TIMP-2 protein and Mouse myeloma Ag8563 cells. |
| 偶联标记 | Un-conjugated |
| 別名 | TIMP-2; Tissue inhibitor of metalloproteinases 2; CSC-21K; DDC8; Metalloproteinase inhibitor 2 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 阳性对照 | IHC: formalin-fixed, paraffin-embedded colonic adenocarcinoma. Western blot: rhTIMP-2, 400 ng per lane. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A/G. |
| 缓冲液 | PBS and 0.08% Sodium azide |
| 抗菌剂 | 0.08% Sodium azide |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | TIMP2 |
| 全名 | TIMP metallopeptidase inhibitor 2 |
| 背景介绍 | This gene is a member of the TIMP gene family. The proteins encoded by this gene family are natural inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix. In addition to an inhibitory role against metalloproteinases, the encoded protein has a unique role among TIMP family members in its ability to directly suppress the proliferation of endothelial cells. As a result, the encoded protein may be critical to the maintenance of tissue homeostasis by suppressing the proliferation of quiescent tissues in response to angiogenic factors, and by inhibiting protease activity in tissues undergoing remodelling of the extracellular matrix. [provided by RefSeq, Jul 2008] |
| 生物功能 | Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-13, MMP-14, MMP-15, MMP-16 and MMP-19. [UniProt] |
| 预测分子量 | 24 kDa |
| 翻译后修饰 | The activity of TIMP2 is dependent on the presence of disulfide bonds. |
检测图片 (3) Click the Picture to Zoom In
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ARG20584 anti-TIMP2 antibody [3A4] WB image
Western blot: 400 ng of 1) TIMP-1, and 2) TIMP-2 recombinant protein stained with ARG20584 anti-TIMP2 antibody [3A4].
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ARG20584 anti-TIMP2 antibody [3A4] IHC-P image
Immunohistochemistry: wax-embedded Human colon stained with ARG20584 anti-TIMP2 antibody [3A4] at 1:32 dilution.
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ARG20584 anti-TIMP2 antibody [3A4] IHC-Wmt image
Immunohistochemistry: whole-mount Zebrafish embryo stained with ARG20584 anti-TIMP2 antibody [3A4].
