ARG54947
anti-TLR9 antibody
anti-TLR9 antibody for ELISA,ICC/IF,Western blot and Human,Mouse,Rat
Immune System antibody; Signaling Transduction antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes TLR9 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ELISA, ICC/IF, WB |
| 特异性 | At least two isoforms of TLR9 are known to exist; this antibody will detect both isoforms. TLR9 antibody is predicted to not cross-react with other TLR proteins. |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | TLR9 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide (15 aa) within aa. 840-890 of Human TLR9. |
| 偶联标记 | Un-conjugated |
| 別名 | CD289; Toll-like receptor 9; CD antigen CD289 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 阳性对照 | Jurkat Cell Lysate | ||||||||
| 实际分子量 | ~ 95 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | PBS and 0.02% Sodium azide |
| 抗菌剂 | 0.02% Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | TLR9 |
| 全名 | toll-like receptor 9 |
| 背景介绍 | TLR9 Antibody: Toll-like receptors (TLRs) are evolutionarily conserved pattern-recognition molecules resembling the toll proteins that mediate antimicrobial responses in Drosophila. These proteins recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. TLR9 forms a subfamily along with TLR7 and TLR8 that recognize viral RNA and CpG DNA sequences and are localized in intracellular acidic compartments such as the phagolysosome. Unlike other TLRs which act through adaptor molecules such as TOLLIP, TIRAP, TRIF, and MyD88 to activate various kinases and transcription factors to respond to potential infection, TLR9 is strictly dependent on MyD88. |
| 生物功能 | Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific to microorganisms. TLR9 is a nucleotide-sensing TLR which is activated by unmethylated cytidine-phosphate-guanosine (CpG) dinucleotides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Controls lymphocyte response to Helicobacter infection. [UniProt] |
| 研究领域 | Immune System antibody; Signaling Transduction antibody |
| 预测分子量 | 116 kDa |
| 翻译后修饰 | Activated by proteolytic cleavage of the flexible loop between repeats LRR14 and LRR15 within the ectodomain. Cleavage requires UNC93B1. Proteolytically processed by first removing the majority of the ectodomain by either asparagine endopeptidase (AEP) or a cathepsin followed by a trimming event that is solely cathepsin mediated and required for optimal receptor signaling. |
检测图片 (3) Click the Picture to Zoom In
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ARG54947 anti-TLR9 antibody WB image
Western blot: Jurkat cell lysate stained with ARG54947 anti-TLR9 antibody at (A) 0.5, (B) 1 and (C) 2 ug/ml dilution.
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ARG54947 anti-TLR9 antibody ICC/IF image
Immunocytochemistry: Jurkat cells stained with ARG54947 anti-TLR9 antibody at 2 ug/ml dilution.
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ARG54947 anti-TLR9 antibody IHC image
Immunohistochemistry: TLR9 in Mouse Spleen tissue stained with ARG54947 anti-TLR9 antibody at 10 ug/ml dilution.
