ARG41854
anti-TRIM63 / MuRF1 antibody [M316]
anti-TRIM63 / MuRF1 antibody [M316] for Western blot and Human
概述
| 产品描述 | Mouse Monoclonal antibody [M316] recognizes TRIM63 / MuRF1 |
|---|---|
| 反应物种 | Hu |
| 应用 | WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | M316 |
| 同位型 | IgG1 |
| 靶点名称 | TRIM63 / MuRF1 |
| 抗原物种 | Human |
| 抗原 | KLH-conjugated synthetic peptide around the C-terminus of Human TRIM63 / MuRF1. |
| 偶联标记 | Un-conjugated |
| 別名 | E3 ubiquitin-protein ligase TRIM63; Muscle-specific RING finger protein 1; EC 6.3.2.-; MURF2; MURF1; MuRF-1; Striated muscle RING zinc finger protein; SMRZ; RNF28; RING finger protein 28; Tripartite motif-containing protein 63; MuRF1; IRF; Iris RING finger protein |
应用说明
| 应用建议 |
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| 应用说明 | WB: Antibody is suggested to be diluted in 5% skimmed milk/Tris buffer with 0.04% Tween20 and incubated for 1 hour at room temperature. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| 实际分子量 | ~ 40 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 缓冲液 | PBS, 0.05% Sodium azide, 50% Glycerol and 1 mg/ml BSA. |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 50% Glycerol and 1 mg/ml BSA |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q969Q1 Human E3 ubiquitin-protein ligase TRIM63 |
|---|---|
| 基因名称 | TRIM63 |
| 全名 | tripartite motif containing 63, E3 ubiquitin protein ligase |
| 背景介绍 | This gene encodes a member of the RING zinc finger protein family found in striated muscle and iris. The product of this gene is an E3 ubiquitin ligase that localizes to the Z-line and M-line lattices of myofibrils. This protein plays an important role in the atrophy of skeletal and cardiac muscle and is required for the degradation of myosin heavy chain proteins, myosin light chain, myosin binding protein, and for muscle-type creatine kinase. [provided by RefSeq, Feb 2012] |
| 生物功能 | E3 ubiquitin ligase. Mediates the ubiquitination and subsequent proteasomal degradation of CKM, GMEB1 and HIBADH. Regulates the proteasomal degradation of muscle proteins under amino acid starvation, where muscle protein is catabolized to provide other organs with amino acids. Inhibits de novo skeletal muscle protein synthesis under amino acid starvation. Regulates proteasomal degradation of cardiac troponin I/TNNI3 and probably of other sarcomeric-associated proteins. May play a role in striated muscle atrophy and hypertrophy by regulating an anti-hypertrophic PKC-mediated signaling pathway. May regulate the organization of myofibrils through TTN in muscle cells. [UniProt] |
| 细胞定位 | Cytoplasm. Nucleus. Cytoplasm, myofibril, sarcomere, M line. Cytoplasm, myofibril, sarcomere, Z line. Note=Colocalizes with TNNI3 in myocytes (By similarity). Localizes to the M- and Z-lines in skeletal muscle. [UniProt] |
| 预测分子量 | 40 kDa |
检测图片 (1) Click the Picture to Zoom In
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ARG41854 anti-TRIM63 / MuRF1 antibody [M316] WB image
Western blot: Human full length MuRF1 recombinant protein stained with ARG41854 anti-TRIM63 / MuRF1 antibody [M316] at 1:250 and 1:1000 dilution.
