ARG66750
anti-TdT antibody [SQab20183]
anti-TdT antibody [SQab20183] for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
概述
| 产品描述 | Recombinant Rabbit Monoclonal antibody [SQab20183] recognizes TdT |
|---|---|
| 反应物种 | Hu |
| 应用 | IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Monoclonal |
| 克隆号 | SQab20183 |
| 同位型 | IgG |
| 靶点名称 | TdT |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide within aa. 1-100 of Human TdT. |
| 偶联标记 | Un-conjugated |
| 別名 | EC 2.7.7.31; Terminal addition enzyme; Terminal transferase; DNA nucleotidylexotransferase; Terminal deoxynucleotidyltransferase; TDT |
应用说明
| 应用建议 |
|
||||||
|---|---|---|---|---|---|---|---|
| 应用说明 | IHC-P: Antigen Retrieval: Heat mediation was performed in Tris/EDTA buffer (pH 9.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
||||||
| 阳性对照 | Thymoma tissue. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A. |
| 缓冲液 | PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA. |
| 抗菌剂 | 0.01% Sodium azide |
| 稳定剂 | 40% Glycerol and 0.05% BSA |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | DNTT |
| 全名 | DNA nucleotidylexotransferase |
| 背景介绍 | This gene is a member of the DNA polymerase type-X family and encodes a template-independent DNA polymerase that catalyzes the addition of deoxynucleotides to the 3'-hydroxyl terminus of oligonucleotide primers. In vivo, the encoded protein is expressed in a restricted population of normal and malignant pre-B and pre-T lymphocytes during early differentiation, where it generates antigen receptor diversity by synthesizing non-germ line elements (N-regions) at the junctions of rearranged Ig heavy chain and T cell receptor gene segments. Alternatively spliced transcript variants encoding different isoforms of this gene have been described. [provided by RefSeq, Jul 2008] |
| 生物功能 | Template-independent DNA polymerase which catalyzes the random addition of deoxynucleoside 5'-triphosphate to the 3'-end of a DNA initiator. One of the in vivo functions of this enzyme is the addition of nucleotides at the junction (N region) of rearranged Ig heavy chain and T-cell receptor gene segments during the maturation of B- and T-cells. [UniProt] |
| 细胞定位 | Nucleus. [UniProt] |
| 预测分子量 | 59 kDa |
检测图片 (2) Click the Picture to Zoom In
-
ARG66750 anti-TdT antibody [SQab20183] WB image (Customer's Feedback)
Western blot: JurKat and Molt-4 cell lysate stained with ARG66750 anti-TdT antibody [SQab20183] at 1:1000 dilution.
-
ARG66750 anti-TdT antibody [SQab20183] IHC-P image
Immunohistochemistry: Formalin/PFA-fixed and paraffin-embedded Human thymoma tissue. Antigen Retrieval: Heat mediation was performed in Tris/EDTA buffer (pH 9.0). The tissue section was stained with ARG66750 anti-TdT antibody [SQab20183] at 18°C - 25°C for 30 minutes.
客户反馈
Excellent
anti-TdT antibody [SQab20183]
Application：WB
Sample：JurKat. Molt4
Sample Loading Amount：20 μg
SDS Page Type：Denatured
Primary Antibody Dilution Factor：1:1000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Conjugation of Secondary Antibody：HRP
