ARG11149
anti-Tyrosine Hydroxylase antibody [4H2]
anti-Tyrosine Hydroxylase antibody [4H2] for ICC/IF,IHC-Frozen sections,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Mouse Monoclonal antibody [4H2] recognizes Tyrosine Hydroxylase |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ICC/IF, IHC-Fr, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 4H2 |
| 同位型 | IgG1 |
| 靶点名称 | Tyrosine Hydroxylase |
| 抗原物种 | Human |
| 抗原 | Full-length Human Tyrosine Hydroxylase. |
| 偶联标记 | Un-conjugated |
| 別名 | DYT14; TYH; Tyrosine 3-monooxygenase; Tyrosine 3-hydroxylase; TH; DYT5b; EC 1.14.16.2 |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 实际分子量 | ~ 60 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified |
| 缓冲液 | PBS, 5 mM Sodium azide and 50% Glycerol. |
| 抗菌剂 | 5 mM Sodium azide |
| 稳定剂 | 50% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | TH |
| 全名 | tyrosine hydroxylase |
| 生物功能 | Plays an important role in the physiology of adrenergic neurons. [UniProt] |
| 预测分子量 | 59 kDa |
检测图片 (2) Click the Picture to Zoom In
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ARG11149 anti-Tyrosine Hydroxylase antibody [4H2] IHC-Fr image
Immunohistochemistry: Frozen section of Rat brain tissue stained with ARG11149 anti-Tyrosine Hydroxylase antibody [4H2] (red) at 1:1000 dilution. Hoechst (blue) for nuclear staining. (Sample preparation: Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45 µM, and free-floating sections were stained with the above antibodies.).
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ARG11149 anti-Tyrosine Hydroxylase antibody [4H2] WB image
Western blot: Rat brain caudate/putamen and PC-12 cell lysates stained with ARG11149 anti-Tyrosine Hydroxylase antibody [4H2] at 1:5000 dilution.
The strong band at about 58 kDa corresponds to TH protein. The weak band at about 80 kDa in the rat brain homogenate is likely an aggregated form of TH, which does not affect the specific cell and process staining of this antibody.
