ARG59199
anti-UBA2 / SAE2 antibody
anti-UBA2 / SAE2 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes UBA2 / SAE2 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | FACS, ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | UBA2 / SAE2 |
| 抗原物种 | Human |
| 抗原 | Recombinant protein corresponding to E449-K564 of Human UBA2 / SAE2. |
| 偶联标记 | Un-conjugated |
| 別名 | EC 6.3.2.-; Anthracycline-associated resistance ARX; ARX; SUMO-activating enzyme subunit 2; SAE2; Ubiquitin-like modifier-activating enzyme 2; Ubiquitin-like 1-activating enzyme E1B; HRIHFB2115 |
应用说明
| 应用建议 |
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| 应用说明 | IHC-P: Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 4% Trehalose. |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 4% Trehalose |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | UBA2 |
| 全名 | ubiquitin-like modifier activating enzyme 2 |
| 背景介绍 | Posttranslational modification of proteins by the addition of the small protein SUMO (see SUMO1; MIM 601912), or sumoylation, regulates protein structure and intracellular localization. SAE1 (MIM 613294) and UBA2 form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins (Okuma et al., 1999 [PubMed 9920803]).[supplied by OMIM, Mar 2010] |
| 生物功能 | The heterodimer acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2. [UniProt] |
| 细胞定位 | Cytoplasm. Nucleus. Note=Shuttles between the cytoplasm and the nucleus, sumoylation is required either for nuclear translocation or nuclear retention. [UniProt] |
| 预测分子量 | 71 kDa |
| 翻译后修饰 | Sumoylated with SUMO1 and SUMO2/3 and by UBC9. Sumoylation at Lys-236 inhibits enzymatic activity. Sumoylation at the C-terminal lysine cluster plays an essential role in nuclear trafficking. [UniProt] |
检测图片 (8) Click the Picture to Zoom In
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ARG59199 anti-UBA2 / SAE2 antibody ICC/IF image
Immunofluorescence: A431 cells were blocked with 10% goat serum and then stained with ARG59199 anti-UBA2 / SAE2 antibody (green) at 2 µg/ml dilution, overnight at 4°C. DAPI (blue) for nuclear staining.
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ARG59199 anti-UBA2 / SAE2 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human colon cancer tissue. Antigen Retrieval: Heat mediated was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG59199 anti-UBA2 / SAE2 antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG59199 anti-UBA2 / SAE2 antibody WB image
Western blot: 50 µg of samples under reducing conditions. HeLa, Human placenta, MCF-7, A549, SK-OV-3, 22RV1, A431 and COLO-320 whole cell lysates stained with ARG59199 anti-UBA2 / SAE2 antibody at 0.5 µg/ml, overnight at 4°C.
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ARG59199 anti-UBA2 / SAE2 antibody FACS image
Flow Cytometry: A431 cells were blocked with 10% normal goat serum and then stained with ARG59199 anti-UBA2 / SAE2 antibody (blue) at 1 µg/10^6 cells for 30 min at 20°C, followed by incubation with DyLight®488 labelled secondary antibody. Isotype control antibody (green) was rabbit IgG (1 µg/10^6 cells) used under the same conditions. Unlabelled sample (red) was also used as a control.
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ARG59199 anti-UBA2 / SAE2 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human mammary cancer tissue. Antigen Retrieval: Heat mediated was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG59199 anti-UBA2 / SAE2 antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG59199 anti-UBA2 / SAE2 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat testis tissue. Antigen Retrieval: Heat mediated was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG59199 anti-UBA2 / SAE2 antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG59199 anti-UBA2 / SAE2 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse testis tissue. Antigen Retrieval: Heat mediated was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG59199 anti-UBA2 / SAE2 antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG59199 anti-UBA2 / SAE2 antibody WB image
Western blot: 50 µg of samples under reducing conditions. Rat brain, Rat lung, Rat liver, Mouse brain, Mouse lung, Mouse liver and Mouse testis lysates stained with ARG59199 anti-UBA2 / SAE2 antibody at 0.5 µg/ml, overnight at 4°C.
