ARG56851
anti-UBE2D1 antibody
anti-UBE2D1 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes UBE2D1 |
|---|---|
| 反应物种 | Hu, Ms |
| 预测物种 | Bov, Rat |
| 应用 | IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | UBE2D1 |
| 抗原物种 | Human |
| 抗原 | KLH-conjugated synthetic peptide corresponding to aa. 111-140 (C-terminus) of Human UBE2D1. |
| 偶联标记 | Un-conjugated |
| 別名 | E2(17)KB1; EC 6.3.2.19; UBC4/5 homolog; Ubiquitin-conjugating enzyme E2; UbcH5; SFT; UBCH5; Ubiquitin carrier protein D1; Ubiquitin-conjugating enzyme E2 D1; UBC4/5; Stimulator of Fe transport; Ubiquitin-protein ligase D1; Ubiquitin-conjugating enzyme E2-17 kDa 1; UBCH5A |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | A431 |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified by ammonium sulfate precipitation through ion exchange chromatography. |
| 缓冲液 | PBS and 0.09% (W/V) Sodium azide. |
| 抗菌剂 | 0.09% (W/V) Sodium azide. |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P51668 Human Ubiquitin-conjugating enzyme E2 D1 Swiss-port # P61080 Mouse Ubiquitin-conjugating enzyme E2 D1 |
|---|---|
| 基因名称 | UBE2D1 |
| 全名 | ubiquitin-conjugating enzyme E2D 1 |
| 背景介绍 | The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is closely related to a stimulator of iron transport (SFT), and is up-regulated in hereditary hemochromatosis. It also functions in the ubiquitination of the tumor-suppressor protein p53 and the hypoxia-inducible transcription factor HIF1alpha by interacting with the E1 ubiquitin-activating enzyme and the E3 ubiquitin-protein ligases. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2011] |
| 生物功能 | Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-linked polyubiquitination. Mediates the selective degradation of short-lived and abnormal proteins. Functions in the E6/E6-AP-induced ubiquitination of p53/TP53. Mediates ubiquitination of PEX5 and auto-ubiquitination of STUB1, TRAF6 and TRIM63/MURF1. Ubiquitinates STUB1-associated HSP90AB1 in vitro. Lacks inherent specificity for any particular lysine residue of ubiquitin. Essential for viral activation of IRF3. Mediates polyubiquitination of CYP3A4. [UniProt] |
| 细胞定位 | Cytoplasm. |
| 预测分子量 | 17 kDa |
检测图片 (2) Click the Picture to Zoom In
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ARG56851 anti-UBE2D1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human liver tissue stained with ARG56851 anti-UBE2D1 antibody at 1:100 dilution.
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ARG56851 anti-UBE2D1 antibody WB image
Western blot: 35 µg of A431 cell lysate stained with ARG56851 anti-UBE2D1 antibody at 1:1000 dilution.
