ARG64919
anti-UBE2F / NCE2 antibody
anti-UBE2F / NCE2 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Cell Biology and Cellular Response antibody; Gene Regulation antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes UBE2F / NCE2 |
|---|---|
| 反应物种 | Hu, Ms |
| 预测物种 | Cow, Rat, Dog |
| 应用 | IHC-P, WB |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | UBE2F / NCE2 |
| 抗原物种 | Human |
| 抗原 | C-HLRDKEDFRNKVDD |
| 偶联标记 | Un-conjugated |
| 別名 | NEDD8-conjugating enzyme 2; EC 6.3.2.-; NEDD8-conjugating enzyme UBE2F; Ubiquitin-conjugating enzyme E2 F; NEDD8 carrier protein UBE2F; NCE2; NEDD8 protein ligase UBE2F |
应用说明
| 应用建议 |
|
||||||
|---|---|---|---|---|---|---|---|
| 应用说明 | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by antigen affinity chromatography. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | UBE2F |
| 全名 | ubiquitin-conjugating enzyme E2F (putative) |
| 生物功能 | Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX2, but not RBX1, suggests that the RBX2-UBE2F complex neddylates specific target proteins, such as CUL5. [UniProt] |
| 研究领域 | Cell Biology and Cellular Response antibody; Gene Regulation antibody |
| 预测分子量 | 21 kDa |
| 翻译后修饰 | The acetylation of Met-1 increases affinity for DCUN1D3 by about 2 orders of magnitude and is crucial for NEDD8 transfer to cullins. |
检测图片 (2) Click the Picture to Zoom In
-
ARG64919 anti-UBE2F / NCE2 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human placenta tissue stained with ARG64919 anti-UBE2F / NCE2 antibody shows membrane staining in trophoblasts. Recommended concentration: 2.5 - 3.8 µg/ml dilution.
-
ARG64919 anti-UBE2F / NCE2 antibody WB image
Western blot: 35 µg of Mouse liver lysate (in RIPA buffer) stained with ARG64919 anti-UBE2F / NCE2 antibody at 0.05 µg/ml dilution and incubated at RT for 1 hour.
