ARG41066
anti-UBE2I / UBC9 antibody
anti-UBE2I / UBC9 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes UBE2I / UBC9 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | FACS, ICC/IF, IHC-P, IP, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | UBE2I / UBC9 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide derived from Human UBE2I / UBC9. |
| 偶联标记 | Un-conjugated |
| 別名 | UBC9; Ubiquitin-protein ligase I; EC 6.3.2.-; SUMO-conjugating enzyme UBC9; Ubiquitin carrier protein I; Ubiquitin-conjugating enzyme E2 I; SUMO-protein ligase; p18; C358B7.1; P18; Ubiquitin carrier protein 9 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.4), 150 mM NaCl, 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | UBE2I |
| 全名 | ubiquitin-conjugating enzyme E2I |
| 背景介绍 | The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Four alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008] |
| 生物功能 | Accepts the ubiquitin-like proteins SUMO1, SUMO2, SUMO3 and SUMO4 from the UBLE1A-UBLE1B E1 complex and catalyzes their covalent attachment to other proteins with the help of an E3 ligase such as RANBP2 or CBX4. Can catalyze the formation of poly-SUMO chains. Necessary for sumoylation of FOXL2 and KAT5. Essential for nuclear architecture and chromosome segregation. Sumoylates p53/TP53 at 'Lys-386'. [UniProt] |
| 细胞定位 | Nucleus. Cytoplasm. Note=Mainly nuclear. In spermatocytes, localizes in synaptonemal complexes. Recruited by BCL11A into the nuclear body (By similarity). [UniProt] |
| 预测分子量 | 18 kDa |
| 翻译后修饰 | Phosphorylation at Ser-71 significantly enhances SUMOylation activity. [UniProt] |
检测图片 (2) Click the Picture to Zoom In
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ARG41066 anti-UBE2I / UBC9 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human kidney stained with ARG41066 anti-UBE2I / UBC9 antibody.
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ARG41066 anti-UBE2I / UBC9 antibody WB image
Western blot: HeLa cell lysate stained with ARG41066 anti-UBE2I / UBC9 antibody.
