ARG55683
anti-UBE2K antibody
anti-UBE2K antibody for ICC/IF,Western blot and Human,Mouse
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes UBE2K |
|---|---|
| 反应物种 | Hu, Ms |
| 预测物种 | Bov |
| 应用 | ICC/IF, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | UBE2K |
| 抗原物种 | Human |
| 抗原 | KLH-conjugated synthetic peptide corresponding to aa. 109-139 (Center) of Human UBE2K. |
| 偶联标记 | Un-conjugated |
| 別名 | 25K; EC 6.3.2.19; LIG; Ubiquitin-conjugating enzyme E2 K; Ubiquitin-conjugating enzyme E2; UBC1; Huntingtin-interacting protein 2; E2-25K; Ubiquitin-conjugating enzyme E2-25 kDa; Ubiquitin-protein ligase; Ubiquitin-conjugating enzyme E2-25K; HYPG; HIP-2; Ubiquitin carrier protein; HIP2 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | 293 |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 缓冲液 | PBS and 0.09% (W/V) Sodium azide. |
| 抗菌剂 | 0.09% (W/V) Sodium azide. |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | UBE2K |
| 全名 | ubiquitin-conjugating enzyme E2K |
| 背景介绍 | The protein encoded by this gene belongs to the ubiquitin-conjugating enzyme family. This protein interacts with RING finger proteins, and it can ubiquitinate huntingtin, the gene product for Huntington's disease. Known functions for this protein include a role in aggregate formation of expanded polyglutamine proteins and the suppression of apoptosis in polyglutamine diseases, a role in the dislocation of newly synthesized MHC class I heavy chains from the endoplasmic reticulum, and involvement in foam cell formation. Multiple transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jul 2008] |
| 生物功能 | Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro, in the presence or in the absence of BRCA1-BARD1 E3 ubiquitin-protein ligase complex, catalyzes the synthesis of 'Lys-48'-linked polyubiquitin chains. Does not transfer ubiquitin directly to but elongates monoubiquitinated substrate protein. Mediates the selective degradation of short-lived and abnormal proteins, such as the endoplasmic reticulum-associated degradation (ERAD) of misfolded lumenal proteins. Ubiquitinates huntingtin. May mediate foam cell formation by the suppression of apoptosis of lipid-bearing macrophages through ubiquitination and subsequence degradation of p53/TP53. Proposed to be involved in ubiquitination and proteolytic processing of NF-kappa-B; in vitro supports ubiquitination of NFKB1. In case of infection by cytomegaloviruses may be involved in the US11-dependent degradation of MHC class I heavy chains following their export from the ER to the cytosol. In case of viral infections may be involved in the HPV E7 protein-dependent degradation of RB1. [UniProt] |
| 细胞定位 | Cytoplasm. |
| 预测分子量 | 22 kDa |
| 翻译后修饰 | Sumoylation at Lys-14 impairs catalytic activity. |
检测图片 (2) Click the Picture to Zoom In
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ARG55683 anti-UBE2K antibody ICC/IF image
Immunofluorescence: HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then stained with ARG55683 anti-UBE2K antibody (green) at 1:25 dilution, 1 hour at 37°C. Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/ml, 1 hour at 37°C).
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ARG55683 anti-UBE2K antibody WB image
Western blot: 35 µg of 293 cell lysate stained with ARG55683 anti-UBE2K antibody.
