ARG63591
anti-UBE2K antibody
anti-UBE2K antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Cell Biology and Cellular Response antibody; Gene Regulation antibody; Neuroscience antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes UBE2K |
|---|---|
| 反应物种 | Hu |
| 预测物种 | Ms, Cow, Dog, Pig |
| 应用 | IHC-P, WB |
| 特异性 | This antibody is expected to recognise isoform 1 (NP_005330.1), isoform 2 (NP_001104582.1) and isoform 3 (NP_001104583.1). |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | UBE2K |
| 抗原物种 | Human |
| 抗原 | C-SWDVETATELLLSN |
| 偶联标记 | Un-conjugated |
| 別名 | 25K; EC 6.3.2.19; LIG; Ubiquitin-conjugating enzyme E2 K; Ubiquitin-conjugating enzyme E2; UBC1; Huntingtin-interacting protein 2; E2-25K; Ubiquitin-conjugating enzyme E2-25 kDa; Ubiquitin-protein ligase; Ubiquitin-conjugating enzyme E2-25K; HYPG; HIP-2; Ubiquitin carrier protein; HIP2 |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|---|---|
| 应用说明 | IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by antigen affinity chromatography. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 背景介绍 | The protein encoded by this gene belongs to the ubiquitin-conjugating enzyme family. This protein interacts with RING finger proteins, and it can ubiquitinate huntingtin, the gene product for Huntington's disease. Known functions for this protein include a role in aggregate formation of expanded polyglutamine proteins and the suppression of apoptosis in polyglutamine diseases, a role in the dislocation of newly synthesized MHC class I heavy chains from the endoplasmic reticulum, and involvement in foam cell formation. Multiple transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jul 2008] |
| 研究领域 | Cell Biology and Cellular Response antibody; Gene Regulation antibody; Neuroscience antibody |
| 预测分子量 | 22 kDa |
| 翻译后修饰 | Sumoylation at Lys-14 impairs catalytic activity. |
检测图片 (2) Click the Picture to Zoom In
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ARG63591 anti-UBE2K antibody WB image
Western blot: Jurkat lysate (RIPA buffer, 35 µg total protein per lane) stained with ARG63591 anti-UBE2K antibody at 1.5 µg/ml dilution.
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ARG63591 anti-UBE2K antibody IHC-P image
Immunohistochemistry: Paraffin embedded Human Cortex. (Steamed antigen retrieval with citrate buffer pH 6) stained with ARG63591 anti-UBE2K antibody at 3.8 µg/ml dilution followed by AP-staining.
