ARG63598
anti-UBE2L3 antibody
anti-UBE2L3 antibody for ICC/IF,Western blot and Human,Mouse,Rat,Pig
Cell Biology and Cellular Response antibody; Gene Regulation antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes UBE2L3 |
|---|---|
| 反应物种 | Hu, Ms, Rat, Pig |
| 预测物种 | Cow |
| 应用 | ICC/IF, WB |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | UBE2L3 |
| 抗原物种 | Human |
| 抗原 | C-EFTKKYGEKRPVD |
| 偶联标记 | Un-conjugated |
| 別名 | UbcM4; Ubiquitin-protein ligase L3; Ubiquitin-conjugating enzyme E2-F1; Ubiquitin carrier protein L3; E2-F1; EC 6.3.2.19; UBCH7; UbcH7; Ubiquitin-conjugating enzyme E2 L3; L-UBC |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|---|---|
| 应用说明 | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by antigen affinity chromatography. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P68036 Human Ubiquitin-conjugating enzyme E2 L3 Swiss-port # P68037 Mouse Ubiquitin-conjugating enzyme E2 L3 |
|---|---|
| 背景介绍 | The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s) and ubiquitin-protein ligases (E3s). This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is demonstrated to participate in the ubiquitination of p53, c-Fos, and the NF-kB precursor p105 in vitro. Several alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Sep 2009] |
| 研究领域 | Cell Biology and Cellular Response antibody; Gene Regulation antibody |
| 预测分子量 | 18 kDa |
| 翻译后修饰 | Ubiquitinated. The alteration of UBE2L3 protein levels during the S-phase of the cell cycle is due to ubiquitin-dependent proteasomal degradation. |
检测图片 (6) Click the Picture to Zoom In
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ARG63598 anti-UBE2L3 antibody WB image
Western Blot: HeLa lysate (RIPA buffer, 35 µg total protein per lane) stained with ARG63598 anti-UBE2L3 antibody at 0.5 µg/ml dilution.
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ARG63598 anti-UBE2L3 antibody ICC/IF image
Immunofluorescence: HeLa cells stained with ARG63598 anti-UBE2L3 antibody (green) at 5 µg/ml dilution. DAPI (blue) for nuclear staining.
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ARG63598 anti-UBE2L3 antibody WB image
Western blot: 35 µg of Human (A), Mouse (B), Rat (C) and Pig (D) heart lysates (in RIPA buffer) stained with ARG63598 anti-UBE2L3 antibody at 0.01 µg/ml dilution and incubated at RT for 1 hour.
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ARG63598 anti-UBE2L3 antibody ICC/IF image
Immunofluorescence: MCF7 cells stained with ARG63598 anti-UBE2L3 antibody (green) at 5 µg/ml dilution. DAPI (blue) for nuclear staining.
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ARG63598 anti-UBE2L3 antibody WB image
Western blot: 35 µg of Human (A), Mouse (B) and Rat (C) skeletal muscle lysates (in RIPA buffer) stained with ARG63598 anti-UBE2L3 antibody at 0.03 µg/ml dilution and incubated at RT for 1 hour.
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ARG63598 anti-UBE2L3 antibody WB image
Western blot: 35 µg of HEK293 (A), HepG2 (B) and Jurkat (C) cell lysates (in RIPA buffer) stained with ARG63598 anti-UBE2L3 antibody at 0.01 µg/ml dilution and incubated at RT for 1 hour.
