ARG45251
anti-UBE2M / UBC12 antibody
anti-UBE2M / UBC12 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Polyclonal antibody recognizes UBE2M / UBC12 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | FACS, ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | UBE2M / UBC12 |
| 抗原物种 | Human |
| 抗原 | Recombinant protein containing to human UBE2M / UBC12. |
| 偶联标记 | Un-conjugated |
| 別名 | UBE2M; Ubiquitin-conjugating enzyme E2 M; UBC-RS2; EC 6.3.2.-; hUbc12; UBC12; NEDD8 protein ligase; NEDD8-conjugating enzyme Ubc12; NEDD8 carrier protein |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| 实际分子量 | 21 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose. |
| 稳定剂 | 4% Trehalose |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | UBE2M |
| 全名 | Ubiquitin Conjugating Enzyme E2 M |
| 背景介绍 | The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein is linked with a ubiquitin-like protein, NEDD8, which can be conjugated to cellular proteins, such as Cdc53/culin. [provided by RefSeq, Jul 2008] |
| 生物功能 | Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX1, but not RBX2, suggests that the RBX1-UBE2M complex neddylates specific target proteins, such as CUL1, CUL2, CUL3 and CUL4. Involved in cell proliferation. [UniProt] |
| 细胞定位 | cytosol; glutamatergic synapse; nucleoplasm; nucleus; postsynapse; presynapse. [UniProt] |
| 预测分子量 | 21 kDa |
| 翻译后修饰 | The acetylation of Met-1 increases affinity for DCUN1D1 by about 2 orders of magnitude and is crucial for NEDD8 transfer to cullins. [UniProt] |
检测图片 (6) Click the Picture to Zoom In
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ARG45251 anti-UBE2M / UBC12 antibody IHC-P image
Immunohistochemistry: Human spleen stained with ARG45251 anti-UBE2M / UBC12 antibody at 2 μg/ml dilution.
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ARG45251 anti-UBE2M / UBC12 antibody ICC/IF image
Immunofluorescence: U87 stained with ARG45251 anti-UBE2M / UBC12 antibody at 5 ug/ml dilution.
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ARG45251 anti-UBE2M / UBC12 antibody WB image
Western blot: 293T and Ramos stained with ARG45251 anti-UBE2M / UBC12 antibody at 0.5 μg/ml dilution.
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ARG45251 anti-UBE2M / UBC12 antibody FACS image
Flow Cytometry: Daudi stained with ARG45251 anti-UBE2M / UBC12 antibody at 1 µg/10^6 cells dilution.
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ARG45251 anti-UBE2M / UBC12 antibody WB image
Western blot: Rat brain stained with ARG45251 anti-UBE2M / UBC12 antibody at 0.5 μg/ml dilution.
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ARG45251 anti-UBE2M / UBC12 antibody WB image
Western blot: Mouse brain stained with ARG45251 anti-UBE2M / UBC12 antibody at 0.5 μg/ml dilution.
