ARG22883
anti-UCHL1 / PGP9.5 antibody [31A3]
anti-UCHL1 / PGP9.5 antibody [31A3] for ELISA,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Rat,Rabbit
概述
| 产品描述 | Mouse Monoclonal antibody [31A3] recognizes UCHL1 / PGP9.5 Mouse anti Human Protein Gene Product 9.5 antibody, clone 31A3 recognizes protein gene product 9.5 (PGP9.5), a ubiquitin hydrolase which is widely expressed in neuronal tissues and represents 1-2% of total soluble brain proteins. PGP9.5, also known as ubiquitin C-terminal hydrolase 1 (UCHL-1), is involved in the regulation of the ubuiquitin pathway.Mouse anti Human Protein Gene Product 9.5 antibody, clone 31A3 stains neuronal cell bodies and axons in the CNS and periphery, small nerve fibres in peripheral tissues, neuroendocrine cells in the pituitary, thyroid, pancreas and tumours of the DNES.Clones 31A3 and 13C4 each recognise a different epitope towards the N-terminus of the protein. Mouse anti Human Protein Gene Product 9.5 antibody, clone 31A3 also recognizes PGP9.5 in other species, including rat and rabbit but evidence suggests it does not bind to PGP9.5 in guinea pigs (Wilson et al. 1988). Mouse anti Human Protein Gene Product 9.5 antibody, clone 31A3 has been used successfully as a capture reagent with clone 13C4 as a detection reagent in a sandwich ELISA to evaluate contamination of processed meat samples with neuronal tissue (Gaunitz et al. 2009). |
|---|---|
| 反应物种 | Hu, Rat, Rb |
| 不反应物种 | Gpig |
| 应用 | ELISA, IHC-P, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 31A3 |
| 同位型 | IgG1 |
| 靶点名称 | UCHL1 / PGP9.5 |
| 抗原物种 | Human |
| 抗原 | Native, from brain |
| 偶联标记 | Un-conjugated |
| 別名 | PGP95; UCH-L1; PGP9.5; PARK5; Ubiquitin thioesterase L1; HEL-117; Neuron cytoplasmic protein 9.5; Uch-L1; EC 6.-.-.-; PGP 9.5; Ubiquitin carboxyl-terminal hydrolase isozyme L1; NDGOA; EC 3.4.19.12 |
应用说明
| 应用建议 |
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| 应用说明 | IHC-P: This antibody is suitable for use on paraffin embedded tissue sections. We do however recommend fixation in 95% ethanol / 5% acetic acid for 2-3 hours prior to paraffin embedding. Specimens which have not been fixed in acetic acid/alcohol will require pretreatment using the microwave-citrate buffer method. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A. |
| 缓冲液 | PBS and 0.09% Sodium azide |
| 抗菌剂 | 0.09% Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P09936 Human Ubiquitin carboxyl-terminal hydrolase isozyme L1 Swiss-port # Q00981 Rat Ubiquitin carboxyl-terminal hydrolase isozyme L1 |
|---|---|
| 基因名称 | UCHL1 |
| 全名 | ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase) |
| 背景介绍 | The protein encoded by this gene belongs to the peptidase C12 family. This enzyme is a thiol protease that hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. This gene is specifically expressed in the neurons and in cells of the diffuse neuroendocrine system. Mutations in this gene may be associated with Parkinson disease.[provided by RefSeq, Sep 2009] |
| 生物功能 | Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity. [UniProt] |
| 预测分子量 | 25 kDa |
| 翻译后修饰 | O-glycosylated. |
检测图片 (1) Click the Picture to Zoom In
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ARG22883 anti-UCHL1 / PGP9.5 antibody [31A3] WB image
Western blot: A549 human alveolar adenocarcinoma whole cell lysate stained with ARG22883 anti-UCHL1 / PGP9.5 antibody [31A3].
