ARG11037
anti-UCHL1 / PGP9.5 antibody
anti-UCHL1 / PGP9.5 antibody for ICC/IF,IHC-Frozen sections,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes UCHL1 / PGP9.5 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 预测物种 | Chk, Cow, Hrs, Pig |
| 应用 | ICC/IF, IHC-Fr, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | UCHL1 / PGP9.5 |
| 抗原物种 | Human |
| 抗原 | Recombinant full length Human UCHL1 purified from E. coli. |
| 偶联标记 | Un-conjugated |
| 別名 | PGP95; UCH-L1; PGP9.5; PARK5; Ubiquitin thioesterase L1; HEL-117; Neuron cytoplasmic protein 9.5; Uch-L1; PGP 9.5; Ubiquitin carboxyl-terminal hydrolase isozyme L1; NDGOA; EC 3.4.19.12 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 实际分子量 | ~ 26 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Unpurified. |
| 缓冲液 | Serum |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | UCHL1 |
| 全名 | ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase) |
| 背景介绍 | The protein encoded by this gene belongs to the peptidase C12 family. This enzyme is a thiol protease that hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. This gene is specifically expressed in the neurons and in cells of the diffuse neuroendocrine system. Mutations in this gene may be associated with Parkinson disease.[provided by RefSeq, Sep 2009] |
| 生物功能 | Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity. [UniProt] |
| 预测分子量 | 25 kDa |
| 翻译后修饰 | O-glycosylated. [UniProt] |
检测图片 (4) Click the Picture to Zoom In
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ARG11037 anti-UCHL1 / PGP9.5 antibody ICC/IF image
Immunofluorescence: SH-SY5Y cells stained with ARG11037 anti-UCHL1 / PGP9.5 antibody (green) at 1:1000 dilution and costained with ARG52280 anti-Fibrillarin antibody [38F3] (red) at 1:1000 dilution. DAPI (blue) for nuclear staining.
The UCHL1 antibody produces strong staining of the cellular cytoplasm of these cells which share many properties with neurons, while the Fibrillarin antibody specifically labels nucleoli.
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ARG11037 anti-UCHL1 / PGP9.5 antibody ICC/IF image
Immunofluorescence: Rat mixed neuron/glial cultures stained with ARG11037 anti-UCHL1 / PGP9.5 antibody (green). Blue is a DNA stain.
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ARG11037 anti-UCHL1 / PGP9.5 antibody WB image
Western blot: Rat brain, Mouse brain, NIH/3T3, HEK293, HeLa and SH-SY5Y cell lysates. The blots were stained with ARG11037 anti-UCHL1 / PGP9.5 antibody (red) at 1:2000 dilution and Mouse mAb to Hsp 60 (green) at 1:10000 dilution.
UCHL1 is detectable in CNS extracts and cells with neuronal properties but not in HeLa, NIH/3T3 and other non-neuronal cells.
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ARG11037 anti-UCHL1 / PGP9.5 antibody WB image
Western blot: 1) Rat brain and 2) Mouse brain lysates stained with ARG11037 anti-UCHL1 / PGP9.5 antibody.
