ARG55836
anti-UCHL1 / PGP9.5 antibody
anti-UCHL1 / PGP9.5 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes UCHL1 / PGP9.5 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 预测物种 | Bov, Mk, Pig |
| 应用 | FACS, ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | UCHL1 / PGP9.5 |
| 抗原物种 | Human |
| 抗原 | KLH-conjugated synthetic peptide corresponding to aa. 187-216 (C-terminus) of Human UCHL1. |
| 偶联标记 | Un-conjugated |
| 別名 | PGP95; UCH-L1; PGP9.5; PARK5; Ubiquitin thioesterase L1; HEL-117; Neuron cytoplasmic protein 9.5; Uch-L1; EC 6.-.-.-; PGP 9.5; Ubiquitin carboxyl-terminal hydrolase isozyme L1; NDGOA; EC 3.4.19.12 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| 阳性对照 | SH-SY5Y |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| 缓冲液 | PBS and 0.09% (W/V) Sodium azide |
| 抗菌剂 | 0.09% (W/V) Sodium azide |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | UCHL1 |
| 全名 | ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase) |
| 背景介绍 | The protein encoded by this gene belongs to the peptidase C12 family. This enzyme is a thiol protease that hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. This gene is specifically expressed in the neurons and in cells of the diffuse neuroendocrine system. Mutations in this gene may be associated with Parkinson disease.[provided by RefSeq, Sep 2009] |
| 生物功能 | Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity. [UniProt] |
| 细胞定位 | Cytoplasm. Endoplasmic reticulum membrane; Lipid-anchor. Note=About 30% of total UCHL1 is associated with membranes in brain |
| 预测分子量 | 25 kDa |
| 翻译后修饰 | O-glycosylated. |
检测图片 (4) Click the Picture to Zoom In
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ARG55836 anti-UCHL1 / PGP9.5 antibody ICC/IF image
Immunofluorescence: NCI-H460 cells stained with ARG55836 anti-UCHL1 / PGP9.5 antibody (green). DAPI (blue) for nuclear staining.
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ARG55836 anti-UCHL1 / PGP9.5 antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human cancer tissue stained with ARG55836 anti-UCHL1 / PGP9.5 antibody.
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ARG55836 anti-UCHL1 / PGP9.5 antibody WB image
Western blot: 35 µg of SH-SY5Y cell lysate stained with ARG55836 anti-UCHL1 / PGP9.5 antibody.
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ARG55836 anti-UCHL1 / PGP9.5 antibody FACS image
Flow Cytometry: NCI-H460 cells stained with ARG55836 anti-UCHL1 / PGP9.5 antibody (right histogram) or without primary antibody control (left histogram), followed by incubation with FITC labelled secondary antibody.
