ARG55604
anti-UCHL3 antibody
anti-UCHL3 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Cell Biology and Cellular Response antibody; Gene Regulation antibody; Neuroscience antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes UCHL3 |
|---|---|
| 反应物种 | Hu |
| 预测物种 | Ms, Rat, Bov, Pig |
| 应用 | IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | UCHL3 |
| 抗原物种 | Human |
| 抗原 | KLH-conjugated synthetic peptide corresponding to aa. 195-225 (C-terminus) of Human UCHL3. |
| 偶联标记 | Un-conjugated |
| 別名 | UCH-L3; Ubiquitin carboxyl-terminal hydrolase isozyme L3; Ubiquitin thioesterase L3; EC 3.4.19.12 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | 293T |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 缓冲液 | PBS and 0.09% (W/V) Sodium azide |
| 抗菌剂 | 0.09% (W/V) Sodium azide |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P15374 Human Ubiquitin carboxyl-terminal hydrolase isozyme L3 |
|---|---|
| 基因名称 | UCHL3 |
| 全名 | ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase) |
| 背景介绍 | The protein encoded by this gene is a member of the deubiquitinating enzyme family. Members of this family are proteases that catalyze the removal of ubiquitin from polypeptides and are divided into five classes, depending on the mechanism of catalysis. This protein may hydrolyze the ubiquitinyl-N-epsilon amide bond of ubiquitinated proteins to regenerate ubiquitin for another catalytic cycle. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Aug 2012] |
| 生物功能 | Deubiquitinating enzyme (DUB) that controls levels of cellular ubiquitin through processing of ubiquitin precursors and ubiquitinated proteins. Thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of either ubiquitin or NEDD8. Has a 10-fold preference for Arg and Lys at position P3", and exhibits a preference towards 'Lys-48'-linked Ubiquitin chains. Deubiquitinates ENAC in apical compartments, thereby regulating apical membrane recycling. Indirectly increases the phosphorylation of IGFIR, AKT and FOXO1 and promotes insulin-signaling and insulin-induced adipogenesis. Required for stress-response retinal, skeletal muscle and germ cell maintenance. May be involved in working memory. Can hydrolyze UBB(+1), a mutated form of ubiquitin which is not effectively degraded by the proteasome and is associated with neurogenerative disorders. [UniProt] |
| 细胞定位 | Cytoplasm. |
| 研究领域 | Cell Biology and Cellular Response antibody; Gene Regulation antibody; Neuroscience antibody |
| 预测分子量 | 26 kDa |
检测图片 (2) Click the Picture to Zoom In
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ARG55604 anti-UCHL3 antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human breast carcinoma tissue stained with ARG55604 anti-UCHL3 antibody.
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ARG55604 anti-UCHL3 antibody WB image
Western blot: 35 µg of 293T cell lysate stained with ARG55604 anti-UCHL3 antibody at 1:1000 dilution.
