ARG53983
anti-UHRF1 antibody (N-term)
anti-UHRF1 antibody (N-term) for Western blot and Human,Mouse
Gene Regulation antibody
概述
| 产品描述 | Mouse Monoclonal antibody recognizes UHRF1 |
|---|---|
| 反应物种 | Hu, Ms |
| 应用 | WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 同位型 | IgG2b |
| 靶点名称 | UHRF1 |
| 抗原物种 | Human |
| 抗原 | Purified recombinant human UHRF1 protein fragments expressed in E.coil. |
| 偶联标记 | Un-conjugated |
| 別名 | Nuclear zinc finger protein Np95; HuNp95; EC 6.3.2.-; hUHRF1; RING finger protein 106; hNp95; Ubiquitin-like PHD and RING finger domain-containing protein 1; Nuclear protein 95; ICBP90; Transcription factor ICBP90; RNF106; Np95; Inverted CCAAT box-binding protein of 90 kDa; Ubiquitin-like-containing PHD and RING finger domains protein 1; huNp95; E3 ubiquitin-protein ligase UHRF1; hNP95 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
| 实际分子量 | 90 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified |
| 缓冲液 | 0.1M Tris-Glycine (pH 7.4), 150 mM NaCl, 0.2% Sodium azide and 50% Glycerol |
| 抗菌剂 | 0.2% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | UHRF1 |
| 全名 | ubiquitin-like with PHD and ring finger domains 1 |
| 背景介绍 | Putative E3 ubiquitin-protein ligase. May participate in methylation-dependent transcriptional regulation. Binds to inverted 5'-CCAAT-3' box 2 in the TOP2A promoter, and activates TOP2A expression. Important for G1/S transition. May be involved in DNA repair and chromosomal stability. |
| 生物功能 | Multidomain protein that acts as a key epigenetic regulator by bridging DNA methylation and chromatin modification. Specifically recognizes and binds hemimethylated DNA at replication forks via its YDG domain and recruits DNMT1 methyltransferase to ensure faithful propagation of the DNA methylation patterns through DNA replication. In addition to its role in maintenance of DNA methylation, also plays a key role in chromatin modification: through its tudor-like regions and PHD-type zinc fingers, specifically recognizes and binds histone H3 trimethylated at 'Lys-9' (H3K9me3) and unmethylated at 'Arg-2' (H3R2me0), respectively, and recruits chromatin proteins. Enriched in pericentric heterochromatin where it recruits different chromatin modifiers required for this chromatin replication. Also localizes to euchromatic regions where it negatively regulates transcription possibly by impacting DNA methylation and histone modifications. Has E3 ubiquitin-protein ligase activity by mediating the ubiquitination of target proteins such as histone H3 and PML. It is still unclear how E3 ubiquitin-protein ligase activity is related to its role in chromatin in vivo. May be involved in DNA repair. [UniProt] |
| 细胞定位 | Nucleus |
| 研究领域 | Gene Regulation antibody |
| 预测分子量 | 90 kDa |
| 翻译后修饰 | Phosphorylation at Ser-298 of the linker region decreases the binding to H3K9me3. Phosphorylation at Ser-639 by CDK1 during M phase impairs interaction with USP7, preventing deubiquitination and leading to degradation by the proteasome. Ubiquitinated; which leads to proteasomal degradation. Autoubiquitinated; interaction with USP7 leads to deubiquitination and prevents degradation. Ubiquitination and degradation takes place during M phase, when phosphorylation at Ser-639 prevents intereaction with USP7 and subsequent deubiquitination. Polyubiquitination may be stimulated by DNA damage. |
检测图片 (1) Click the Picture to Zoom In
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ARG53983 anti-UHRF1 antibody (N-term) WB image
Western blot: Mouse heart lysate stained with ARG53983 anti-UHRF1 antibody (N-term) at 1:1000 dilution.
