ARG55818
anti-USP11 / UHX1 antibody [1220CT620.193.189]
anti-USP11 / UHX1 antibody [1220CT620.193.189] for Flow cytometry,Western blot and Human
概述
| 产品描述 | Mouse Monoclonal antibody recognizes USP11 / UHX1 |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 1220CT620.193.189 |
| 同位型 | IgG1, kappa |
| 靶点名称 | USP11 / UHX1 |
| 抗原物种 | Human |
| 抗原 | KLH-conjugated synthetic peptide corresponding to aa. 32-300 (C-terminus) of Human USP11. |
| 偶联标记 | Un-conjugated |
| 別名 | UHX1; Deubiquitinating enzyme 11; Ubiquitin thioesterase 11; Ubiquitin carboxyl-terminal hydrolase 11; Ubiquitin-specific-processing protease 11; EC 3.4.19.12 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | Jurkat |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 缓冲液 | PBS and 0.09% (W/V) Sodium azide |
| 抗菌剂 | 0.09% (W/V) Sodium azide |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P51784 Human Ubiquitin carboxyl-terminal hydrolase 11 |
|---|---|
| 基因名称 | USP11 |
| 全名 | ubiquitin specific peptidase 11 |
| 背景介绍 | Protein ubiquitination controls many intracellular processes, including cell cycle progression, transcriptional activation, and signal transduction. This dynamic process, involving ubiquitin conjugating enzymes and deubiquitinating enzymes, adds and removes ubiquitin. Deubiquitinating enzymes are cysteine proteases that specifically cleave ubiquitin from ubiquitin-conjugated protein substrates. This gene encodes a deubiquitinating enzyme which lies in a gene cluster on chromosome Xp11.23 [provided by RefSeq, Jul 2008] |
| 生物功能 | Protease that can remove conjugated ubiquitin from target proteins and polyubiquitin chains. Inhibits the degradation of target proteins by the proteasome. Plays a role in the regulation of pathways leading to NF-kappa-B activation. Plays a role in the regulation of DNA repair after double-stranded DNA breaks. [UniProt] |
| 细胞定位 | Nucleus. Cytoplasm. Note=Predominantly nuclear. Associates with chromatin |
| 预测分子量 | 110 kDa |
检测图片 (2) Click the Picture to Zoom In
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ARG55818 anti-USP11 / UHX1 antibody WB image
Western blot: 35 µg of Jurkat cell lysate stained with ARG55818 anti-USP11 / UHX1 antibody at 1:1000 dilution.
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ARG55818 anti-USP11 / UHX1 antibody FACS image
Flow Cytometry: HeLa cells stained with ARG55818 anti-USP11 / UHX1 antibody (right histogram) at 1:25 dilution or isotype control antibody (left histogram), followed by incubation with Alexa Fluor® 488 labelled secondary antibody.
