概述

产品描述	Rabbit Polyclonal antibody recognizes USP13
反应物种	Hu, Ms
应用	FACS, ICC/IF, IP, WB
宿主	Rabbit
克隆	Polyclonal
同位型	IgG
靶点名称	USP13
抗原物种	Human
抗原	Synthetic peptide of Human USP13.
偶联标记	Un-conjugated
別名	Isopeptidase T-3; Ubiquitin-specific-processing protease 13; Deubiquitinating enzyme 13; ISOT3; IsoT-3; Ubiquitin thioesterase 13; Ubiquitin carboxyl-terminal hydrolase 13; ISOT-3; EC 3.4.19.12

应用说明

应用建议	应用 推荐稀释比 FACS 1:100 ICC/IF 1:50 - 1:200 IP 1:50 WB 1:500 - 1:2000
应用说明	* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
阳性对照	HepG2
实际分子量	~ 95 kDa

属性

形式	Liquid
纯化	Affinity purified.
缓冲液	PBS (pH 7.4), 150 mM NaCl, 0.02% Sodium azide and 50% Glycerol.
抗菌剂	0.02% Sodium azide
稳定剂	50% Glycerol
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 72607 Mouse USP13 GeneID: 8975 Human USP13 Swiss-port # Q5BKP2 Mouse Ubiquitin carboxyl-terminal hydrolase 13 Swiss-port # Q92995 Human Ubiquitin carboxyl-terminal hydrolase 13
基因名称	USP13
全名	ubiquitin specific peptidase 13 (isopeptidase T-3)
生物功能	Deubiquitinase that mediates deubiquitination of target proteins such as BECN1, MITF, SKP2 and USP10 and is involved in various processes such as autophagy and endoplasmic reticulum-associated degradation (ERAD). Component of a regulatory loop that controls autophagy and p53/TP53 levels: mediates deubiquitination of BECN1, a key regulator of autophagy, leading to stabilize the PIK3C3/VPS34-containing complexes. Also deubiquitinates USP10, an essential regulator of p53/TP53 stability. In turn, PIK3C3/VPS34-containing complexes regulate USP13 stability, suggesting the existence of a regulatory system by which PIK3C3/VPS34-containing complexes regulate p53/TP53 protein levels via USP10 and USP13. Recruited by nuclear UFD1 and mediates deubiquitination of SKP2, thereby regulating endoplasmic reticulum-associated degradation (ERAD). Mediates stabilization of SIAH2 independently of deubiquitinase activity: binds ubiquitinated SIAH2 and acts by impairing SIAH2 autoubiquitination. Has a weak deubiquitinase activity in vitro and preferentially cleaves 'Lys-63'-linked polyubiquitin chains. In contrast to USP5, it is not able to mediate unanchored polyubiquitin disassembly. Able to cleave ISG15 in vitro; however, additional experiments are required to confirm such data. [UniProt]
预测分子量	97 kDa

检测图片 (1) Click the Picture to Zoom In