概述

产品描述	Rabbit Polyclonal antibody recognizes USP14
反应物种	Hu, Ms, Rat
应用	FACS, ICC/IF, WB
宿主	Rabbit
克隆	Polyclonal
同位型	IgG
靶点名称	USP14
抗原物种	Human
抗原	Synthetic peptide of Human USP14.
偶联标记	Un-conjugated
別名	Deubiquitinating enzyme 14; TGT; Ubiquitin thioesterase 14; Ubiquitin-specific-processing protease 14; Ubiquitin carboxyl-terminal hydrolase 14; EC 3.4.19.12

应用说明

应用建议	应用 推荐稀释比 FACS 1:60 ICC/IF 1:50 - 1:200 WB 1:500 - 1:2000
应用说明	* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
阳性对照	HeLa
实际分子量	~ 57 kDa

属性

形式	Liquid
纯化	Affinity purified.
缓冲液	PBS (pH 7.4), 150 mM NaCl, 0.02% Sodium azide and 50% Glycerol.
抗菌剂	0.02% Sodium azide
稳定剂	50% Glycerol
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 59025 Mouse USP14 GeneID: 9097 Human USP14 Swiss-port # P54578 Human Ubiquitin carboxyl-terminal hydrolase 14 Swiss-port # Q9JMA1 Mouse Ubiquitin carboxyl-terminal hydrolase 14
基因名称	USP14
全名	ubiquitin specific peptidase 14 (tRNA-guanine transglycosylase)
背景介绍	This gene encodes a member of the ubiquitin-specific processing (UBP) family of proteases that is a deubiquitinating enzyme (DUB) with His and Cys domains. This protein is located in the cytoplasm and cleaves the ubiquitin moiety from ubiquitin-fused precursors and ubiquitinylated proteins. Mice with a mutation that results in reduced expression of the ortholog of this protein are retarded for growth, develop severe tremors by 2 to 3 weeks of age followed by hindlimb paralysis and death by 6 to 10 weeks of age. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]
生物功能	Proteasome-associated deubiquitinase which releases ubiquitin from the proteasome targeted ubiquitinated proteins. Ensures the regeneration of ubiquitin at the proteasome. Is a reversibly associated subunit of the proteasome and a large fraction of proteasome-free protein exists within the cell. Required for the degradation of the chemokine receptor CXCR4 which is critical for CXCL12-induced cell chemotaxis. Serves also as a physiological inhibitor of endoplasmic reticulum-associated degradation (ERAD) under the non-stressed condition by inhibiting the degradation of unfolded endoplasmic reticulum proteins via interaction with ERN1. Indispensable for synaptic development and function at neuromuscular junctions (NMJs). [UniProt]
细胞定位	Cytoplasm. Cell membrane; Peripheral membrane protein. [UniProt]
预测分子量	56 kDa

检测图片 (1) Click the Picture to Zoom In