ARG55491
anti-USP16 / UBP-M antibody
anti-USP16 / UBP-M antibody for Western blot and Human,Mouse,Rat,Monkey
Cell Biology and Cellular Response antibody; Gene Regulation antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes USP16 / UBP-M |
|---|---|
| 反应物种 | Hu, Ms, Rat, Mk |
| 应用 | WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | USP16 / UBP-M |
| 抗原物种 | Human |
| 抗原 | Recombinant protein of Human USP16 |
| 偶联标记 | Un-conjugated |
| 別名 | UBP-M; Deubiquitinating enzyme 16; Ubiquitin-processing protease UBP-M; Ubiquitin thioesterase 16; Ubiquitin-specific-processing protease 16; UBPM; EC 3.4.19.12; Ubiquitin carboxyl-terminal hydrolase 16 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
| 阳性对照 | Mouse spleen | ||||
| 实际分子量 | 100 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | USP16 |
| 全名 | ubiquitin specific peptidase 16 |
| 背景介绍 | This gene encodes a deubiquitinating enzyme that is phosphorylated at the onset of mitosis and then dephosphorylated at the metaphase/anaphase transition. It can deubiquitinate H2A, one of two major ubiquitinated proteins of chromatin, in vitro and a mutant form of the protein was shown to block cell division. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008] |
| 生物功能 | Specifically deubiquitinates 'Lys-120' of histone H2A (H2AK119Ub), a specific tag for epigenetic transcriptional repression, thereby acting as a coactivator. Deubiquitination of histone H2A is a prerequisite for subsequent phosphorylation at 'Ser-11' of histone H3 (H3S10ph), and is required for chromosome segregation when cells enter into mitosis. In resting B- and T-lymphocytes, phosphorylation by AURKB leads to enhance its activity, thereby maintaining transcription in resting lymphocytes. Regulates Hox gene expression via histone H2A deubiquitination. Prefers nucleosomal substrates. Does not deubiquitinate histone H2B. [UniProt] |
| 研究领域 | Cell Biology and Cellular Response antibody; Gene Regulation antibody |
| 预测分子量 | 94 kDa |
| 翻译后修饰 | Phosphorylated at the onset of mitosis and dephosphorylated during the metaphase/anaphase transition. Phosphorylation by AURKB enhances the deubiquitinase activity. |
检测图片 (1) Click the Picture to Zoom In
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ARG55491 anti-USP16 / UBP-M antibody WB image
Western blot: Mouse spleen lysate stained with ARG55491 anti-USP16 / UBP-M antibody.
