ARG63361
anti-USP20 / VDU2 antibody
anti-USP20 / VDU2 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Cell Biology and Cellular Response antibody; Gene Regulation antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes USP20 / VDU2 |
|---|---|
| 反应物种 | Hu |
| 预测物种 | Ms, Rat, Cow, Dog, Pig |
| 应用 | IHC-P, WB |
| 特异性 | Reported variants represent identical protein (NP_006667.3; NP_001103773.2; NP_001008563.2). |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | USP20 / VDU2 |
| 抗原物种 | Human |
| 抗原 | C-HGEQKIEAETRAV |
| 偶联标记 | Un-conjugated |
| 別名 | Ubiquitin carboxyl-terminal hydrolase 20; LSFR3A; VDU2; VHL-interacting deubiquitinating enzyme 2; Ubiquitin-specific-processing protease 20; hVDU2; Deubiquitinating enzyme 20; Ubiquitin thioesterase 20; EC 3.4.19.12 |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|---|---|
| 应用说明 | IHC-P: Antigen Retrieval: Microwaved tissue section in Tris/EDTA buffer (pH 9.0). WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by antigen affinity chromatography. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q9Y2K6 Human Ubiquitin carboxyl-terminal hydrolase 20 |
|---|---|
| 背景介绍 | This gene encodes a ubiquitin specific processing protease that was first identified as a substrate of the VHL (von Hippel-Lindau disease) protein E3 ubiquitin ligase complex. In addition to being ubiquitinated by the VHL-E3 ligase complex, this enzyme deubiquitinates hypoxia-inducible factor (HIF)-1 alpha and thereby causes increased expression of HIF-1alpha targeted genes which play a role in angiogenesis, glucose metabolism, cell proliferation and metastasis. The enzyme encoded by this gene also regulates G-protein coupled receptor signaling by mediating the deubiquitination of beta-2 adrenergic receptor (ADRB2). This enzyme is a ubiquitously expressed thiolester hydrolase. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Jan 2013] |
| 研究领域 | Cell Biology and Cellular Response antibody; Gene Regulation antibody |
| 预测分子量 | 102 kDa |
| 翻译后修饰 | Ubiquitinated via a VHL-dependent pathway for proteasomal degradation. |
检测图片 (2) Click the Picture to Zoom In
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ARG63361 anti-USP20 / VDU2 antibody IHC-P image
Immunohistochemistry: paraffin embedded Human Lymph Node. (Microwaved antigen retrieval with Tris/EDTA buffer pH9) stained with ARG63361 anti-USP20 / VDU2 antibody at 3 µg/ml dilution followed by HRP-staining.
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ARG63361 anti-USP20 / VDU2 antibody WB image
Western blot: 35 µg of Human Tonsil lysate (in RIPA buffer) stained with ARG63361 anti-USP20 / VDU2 antibody at 1 µg/ml dilution and incubated at RT for 1 hour.
