ARG55001
anti-USP25 antibody [1277CT376.106.171]
anti-USP25 antibody [1277CT376.106.171] for Western blot and Human
Cell Biology and Cellular Response antibody; Gene Regulation antibody
概述
| 产品描述 | Mouse Monoclonal antibody recognizes USP25 |
|---|---|
| 反应物种 | Hu |
| 应用 | WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 1277CT376.106.171 |
| 同位型 | IgG1, kappa |
| 靶点名称 | USP25 |
| 抗原物种 | Human |
| 抗原 | Recombinant protein from Human USP25. |
| 偶联标记 | Un-conjugated |
| 別名 | USP on chromosome 21; Ubiquitin-specific-processing protease 25; Ubiquitin carboxyl-terminal hydrolase 25; Deubiquitinating enzyme 25; Ubiquitin thioesterase 25; USP21; EC 3.4.19.12 |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
| 阳性对照 | Daudi |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 缓冲液 | PBS and 0.09% (W/V) Sodium azide |
| 抗菌剂 | 0.09% (W/V) Sodium azide |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q9UHP3 Human Ubiquitin carboxyl-terminal hydrolase 25 |
|---|---|
| 基因名称 | USP25 |
| 全名 | ubiquitin specific peptidase 25 |
| 背景介绍 | Ubiquitin (MIM 191339) is a highly conserved 76-amino acid protein involved in regulation of intracellular protein breakdown, cell cycle regulation, and stress response. Ubiquitin is released from degraded proteins by disassembly of the polyubiquitin chains, which is mediated by ubiquitin-specific proteases (USPs), such as USP25 (Valero et al., 1999 [PubMed 10644437]).[supplied by OMIM, Mar 2008] |
| 生物功能 | Deubiquitinating enzyme that hydrolyzes ubiquitin moieties conjugated to substrates and thus, functions to process newly synthesized Ubiquitin, to recycle ubiquitin molecules or to edit polyubiquitin chains and prevents proteasomal degradation of substrates. Hydrolyzes both 'Lys-48'- and 'Lys-63'-linked tetraubiquitin chains. The muscle-specific isoform (USP25m) may have a role in the regulation of muscular differentiation and function. [UniProt] |
| 细胞定位 | Cytoplasm |
| 研究领域 | Cell Biology and Cellular Response antibody; Gene Regulation antibody |
| 预测分子量 | 122 kDa |
| 翻译后修饰 | Acetylated. Sumoylation impairs binding to and hydrolysis of ubiquitin chains. Sumoylated preferentially with SUMO2 or SUMO3. Desumoylated by SENP1. Regulated by ubiquitination on the same residue. Preferentially monoubiquitinated but can also be polyubiquitinated. Autodeubiquitinated. Ubiquitination activates the enzymatic activity either by preventing sumoylation or by allowing novel interactions. Phosphorylation in the C-terminal by SYK regulates USP25 cellular levels. |
检测图片 (1) Click the Picture to Zoom In
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ARG55001 anti-USP25 antibody WB image
Western blot: 35 µg of Daudi cell lysate stained with ARG55001 anti-USP25 antibody at 1:1000 dilution.
