ARG54950
anti-USP25 antibody
anti-USP25 antibody for ELISA,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Cell Biology and Cellular Response antibody; Gene Regulation antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes USP25 |
|---|---|
| 反应物种 | Hu, Ms |
| 应用 | ELISA, ICC/IF, IHC-P, WB |
| 特异性 | USP25 antibody is human and mouse reactive. Multiple isoforms of USP25 are known to exist. |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | USP25 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide (17 aa) within aa. 470-520 of Human USP25. |
| 偶联标记 | Un-conjugated |
| 別名 | USP on chromosome 21; Ubiquitin-specific-processing protease 25; Ubiquitin carboxyl-terminal hydrolase 25; Deubiquitinating enzyme 25; Ubiquitin thioesterase 25; USP21; EC 3.4.19.12 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| 阳性对照 | Mouse Brain Tissue Lysate |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | PBS and 0.02% Sodium azide |
| 抗菌剂 | 0.02% Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P57080 Mouse Ubiquitin carboxyl-terminal hydrolase 25 Swiss-port # Q9UHP3 Human Ubiquitin carboxyl-terminal hydrolase 25 |
|---|---|
| 基因名称 | USP25 |
| 全名 | ubiquitin specific peptidase 25 |
| 背景介绍 | USP25 (ubiquitin specific peptidase 25), also known as USP21, belongs to the peptidase C19 family and is a highly conserved 76-amino acid protein involved in regulation of intracellular protein breakdown, cell cycle regulation, and stress response. It contains one UBA-like domain and two UIM (ubiquitin-interacting motif) repeats. Due to alternative splicing events, USP25 is expressed as two short, ubiquitously expressed isoforms and one long, muscle-specific isoform. The long isoform of USP25 (USP25m) is upregulated in myogenesis and is implicated in regulation of muscular differentiation and function. USP25 is a deubiquitinating enzyme (DUB) that negatively regulates IL-17-triggered signaling. |
| 生物功能 | Deubiquitinating enzyme that hydrolyzes ubiquitin moieties conjugated to substrates and thus, functions to process newly synthesized Ubiquitin, to recycle ubiquitin molecules or to edit polyubiquitin chains and prevents proteasomal degradation of substrates. Hydrolyzes both 'Lys-48'- and 'Lys-63'-linked tetraubiquitin chains. The muscle-specific isoform (USP25m) may have a role in the regulation of muscular differentiation and function. [UniProt] |
| 研究领域 | Cell Biology and Cellular Response antibody; Gene Regulation antibody |
| 预测分子量 | 122 kDa |
| 翻译后修饰 | Acetylated. Sumoylation impairs binding to and hydrolysis of ubiquitin chains. Sumoylated preferentially with SUMO2 or SUMO3. Desumoylated by SENP1. Regulated by ubiquitination on the same residue. Preferentially monoubiquitinated but can also be polyubiquitinated. Autodeubiquitinated. Ubiquitination activates the enzymatic activity either by preventing sumoylation or by allowing novel interactions. Phosphorylation in the C-terminal by SYK regulates USP25 cellular levels. |
检测图片 (3) Click the Picture to Zoom In
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ARG54950 anti-USP25 antibody WB image
Western blot: mouse brain tissue lysate stained with ARG54950 anti-USP25 antibody at 1 ug/ml dilution in (Lane 1) the absence and (Lane 2) the presence of blocking peptide.
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ARG54950 anti-USP25 antibody IHC image
Immunohistochemistry: USP25 in human brain tissue stained with ARG54950 anti-USP25 antibody at 20 ug/ml dilution.
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ARG54950 anti-USP25 antibody IHC image
Immunohistochemistry: USP25 in human brain tissue stained with ARG54950 anti-USP25 antibody at 5 ug/ml dilution.
