ARG11036

anti-VE Cadherin antibody

Datasheet

参考文献1

订购信息

包装

价格 (USD)

50 μg

335

arigo in CHINA:
恩瑞科生物
☎ 400-886-0853,
0571-8708-1890

概述

产品描述	Rabbit Polyclonal antibody recognizes VE Cadherin
反应物种	Hu, Ms, Rat
应用	ICC/IF, IHC-P, WB
宿主	Rabbit
克隆	Polyclonal
同位型	IgG
靶点名称	VE Cadherin
抗原物种	Human
抗原	Synthetic peptide around aa. 766-784 of Human VE-Cadherin. (RFKMLAELYGSDPREELLY)
偶联标记	Un-conjugated
別名	7B4 antigen; 7B4; Cadherin-5; VE-cadherin; CD144; CD antigen CD144; Vascular endothelial cadherin

应用说明

应用建议

应用	推荐稀释比
ICC/IF	Assay-dependent
IHC-P	2 - 5 µg/ml
WB	0.1 - 0.5 µg/ml

应用说明

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式	Liquid
纯化	Affinity purification with immunogen.
缓冲液	PBS, 0.025% Sodium azide and 2.5% BSA.
抗菌剂	0.025% Sodium azide
稳定剂	2.5% BSA
浓度	0.5 mg/ml
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 1003 Human CDH5 GeneID: 12562 Mouse CDH5 Swiss-port # P33151 Human Cadherin-5 Swiss-port # P55284 Mouse Cadherin-5
基因名称	CDH5
全名	cadherin 5, type 2 (vascular endothelium)
背景介绍	This gene is a classical cadherin from the cadherin superfamily and is located in a six-cadherin cluster in a region on the long arm of chromosome 16 that is involved in loss of heterozygosity events in breast and prostate cancer. The encoded protein is a calcium-dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Functioning as a classic cadherin by imparting to cells the ability to adhere in a homophilic manner, the protein may play an important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. An alternative splice variant has been described but its full length sequence has not been determined. [provided by RefSeq, Jul 2008]
生物功能	Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. This cadherin may play a important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. It associates with alpha-catenin forming a link to the cytoskeleton. Acts in concert with KRIT1 to establish and maintain correct endothelial cell polarity and vascular lumen. These effects are mediated by recruitment and activation of the Par polarity complex and RAP1B. Required for activation of PRKCZ and for the localization of phosphorylated PRKCZ, PARD3, TIAM1 and RAP1B to the cell junction. [UniProt]
细胞定位	Cell membrane; Cell junction (Note: Found at cell-cell boundaries and probably at cell-matrix boundaries. KRIT1 and CDH5 reciprocally regulate their localization to endothelial cell-cell junctions.) [UniProt]
预测分子量	88 kDa (unmodified); 90 - 140 kDa (glycosylated)
翻译后修饰	Phosphorylated on tyrosine residues by KDR/VEGFR-2. Dephosphorylated by PTPRB (By similarity). O-glycosylated. [UniProt]

检测图片 (3) Click the Picture to Zoom In

ARG11036 anti-VE Cadherin antibody IHC-P image

Immunohistochemistry: Paraffin-embedded Human lung cancer tissue stained with ARG11036 anti-VE Cadherin antibody.
ARG11036 anti-VE Cadherin antibody WB image

Western blot: 1) A549, 2) HeLa and 3) MCF-7 cell lysates stained with ARG11036 anti-VE Cadherin antibody.
ARG11036 anti-VE Cadherin antibody IHC-P image

Immunohistochemistry: Paraffin-embedded Rat brain tissue stained with ARG11036 anti-VE Cadherin antibody.