ARG42065
anti-VEGF antibody (Biotin)
anti-VEGF antibody (Biotin) for ELISA,Western blot and Human
概述
| 产品描述 | Biotin-conjugated Goat Polyclonal antibody recognizes VEGF |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA, WB |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | VEGF |
| 抗原物种 | Human |
| 抗原 | Recombinant Human VEGF. |
| 偶联标记 | Biotin |
| 別名 | MVCD1; Vascular permeability factor; VEGF-A; VPF; VEGF; Vascular endothelial growth factor A |
应用说明
| 应用建议 |
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| 应用说明 | Sandwich ELISA (Capture antibody - Detection antibody): ARG42064 (0.5 - 2 µg/ml) - ARG42065 (0.25 - 1 µg/ml) * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.2) |
| 浓度 | 1 mg/ml |
| 存放说明 | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P15692 Human Vascular endothelial growth factor A |
|---|---|
| 基因名称 | VEGFA |
| 全名 | vascular endothelial growth factor A |
| 生物功能 | Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth. Binding to NRP1 receptor initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development (By similarity). [UniProt] |
| 细胞定位 | Secreted. Note=VEGF121 is acidic and freely secreted. VEGF165 is more basic, has heparin-binding properties and, although a signicant proportion remains cell-associated, most is freely secreted. VEGF189 is very basic, it is cell-associated after secretion and is bound avidly by heparin and the extracellular matrix, although it may be released as a soluble form by heparin, heparinase or plasmin. [UniProt] |
| 产品亮点 | Related products: VEGF antibodies; VEGF ELISA Kits; VEGF Duos / Panels; VEGF recombinant proteins; Anti-Goat IgG secondary antibodies; Related news: The role of HDGF in tumor angiogenesis |
| 预测分子量 | 22 kDa |
检测图片 (1) Click the Picture to Zoom In
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ARG42065 anti-VEGF antibody (Biotin) ELISA image
Sandwich ELISA: Detecting human VEGF (using 100 µl/well antibody solution). ARG42064 anti-VEGF antibody as a capture antibody at 0.5 - 2 µg/ml dilution in combination with ARG42065 anti-VEGF antibody (Biotin) as a detection antibody at 0.25 - 1.0 µg/ml dilution. Results of a typical standard run with optical density reading at 405 - 650 nm.
