ARG45245
anti-VPS53 antibody
anti-VPS53 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Polyclonal antibody recognizes VPS53 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | FACS, ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | VPS53 |
| 抗原物种 | Human |
| 抗原 | Recombinant protein containing to human VPS53. |
| 偶联标记 | Un-conjugated |
| 別名 | VPS53; VPS53 Subunit Of GARP Complex; HCCS1; Vacuolar Protein Sorting-Associated Protein 53 Homolog; Hepatocellular Carcinoma Suppressor 1; VPS53, GARP Complex Subunit; FLJ10979; Vacuolar Protein Sorting 53 Homolog (S. Cerevisiae); Vacuolar Protein Sorting 53 (Yeast); HVps53L; Pp13624; PCH2E |
应用说明
| 应用建议 |
|
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| 实际分子量 | 100 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose. |
| 稳定剂 | 4% Trehalose |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q5VIR6 Human Vacuolar protein sorting-associated protein 53 homolog Swiss-port # Q8CCB4 Mouse Vacuolar protein sorting-associated protein 53 homolog |
|---|---|
| 基因名称 | VPS53 |
| 全名 | VPS53 Subunit Of GARP Complex |
| 背景介绍 | Involved in endocytic recycling and retrograde transport, endosome to Golgi. Acts upstream of or within lysosomal transport. Located in several cellular components, including Golgi apparatus; perinuclear region of cytoplasm; and recycling endosome. Part of EARP complex and GARP complex. Implicated in pontocerebellar hypoplasia type 2E. [provided by Alliance of Genome Resources, Nov 2024] |
| 生物功能 | Acts as a component of the GARP complex that is involved in retrograde transport from early and late endosomes to the trans-Golgi network (TGN). The GARP complex is required for the maintenance of the cycling of mannose 6-phosphate receptors between the TGN and endosomes, this cycling is necessary for proper lysosomal sorting of acid hydrolases such as CTSD. [UniProt] |
| 细胞定位 | Endosome ; Golgi apparatus ; Membrane. [UniProt] |
| 预测分子量 | 94 kDa |
| 翻译后修饰 | Acetylation ; Phosphoprotein. [UniProt] |
检测图片 (6) Click the Picture to Zoom In
-
ARG45245 anti-VPS53 antibody IHC-P image
Immunohistochemistry: Human lung adenocarcinoma stained with ARG45245 anti-VPS53 antibody at 2 μg/ml dilution.
-
ARG45245 anti-VPS53 antibody ICC/IF image
Immunofluorescence: A549 stained with ARG45245 anti-VPS53 antibody at 5 ug/ml dilution.
-
ARG45245 anti-VPS53 antibody WB image
Western blot: HepG2 and MCF-7 stained with ARG45245 anti-VPS53 antibody at 0.5 μg/ml dilution.
-
ARG45245 anti-VPS53 antibody FACS image
Flow Cytometry: U87 stained with ARG45245 anti-VPS53 antibody at 1 µg/10^6 cells dilution.
-
ARG45245 anti-VPS53 antibody IHC-P image
Immunohistochemistry: Rat brain stained with ARG45245 anti-VPS53 antibody at 2 μg/ml dilution.
-
ARG45245 anti-VPS53 antibody IHC-P image
Immunohistochemistry: Mouse brain stained with ARG45245 anti-VPS53 antibody at 2 μg/ml dilution.
