ARG55540

Name: anti-Vimentin antibody
Brand: arigo Biolaboratories
SKU: ARG55540
Price: 399 USD
Availability: InStock
Rating: 4.4 (5 reviews)

anti-Vimentin antibody

Datasheet

订购信息

包装

价格 (USD)

100 μl

399

50 μl

279

arigo in CHINA:
恩瑞科生物
☎ 400-886-0853,
0571-8708-1890

概述

产品描述	Mouse Monoclonal antibody recognizes Vimentin
反应物种	Hu, Mk
应用	ICC/IF, IP, WB
特异性	This antibody detects endogenous levels of Vimentin and does not cross-react with related proteins.
宿主	Mouse
克隆	Monoclonal
同位型	IgG2a
靶点名称	Vimentin
抗原物种	Human
抗原	Purified recombinant fragment of Human Vimentin protein (NP_003371.2).
偶联标记	Un-conjugated
別名	Vimentin; CTRCT30; HEL113

应用说明

应用建议

应用	推荐稀释比
ICC/IF	1:800
IP	Assay-dependent
WB	1:1000

应用说明

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式	Liquid
纯化	Affinity purification with immunogen.
缓冲液	PBS (pH 7.4), 0.03% Proclin 300 and 50% Glycerol
抗菌剂	0.03% Proclin 300
稳定剂	50% Glycerol
浓度	1.64 mg/ml
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 7431 Human VIM Swiss-port # P08670 Human Vimentin
基因名称	VIM
全名	vimentin
背景介绍	Vimentin is a type III intermediate filament protein. Intermediate filaments, along with microtubules and actin microfilaments, make up the cytoskeleton. The encoded protein is responsible for maintaining cell shape and integrity of the cytoplasm, and stabilizing cytoskeletal interactions. This protein is involved in neuritogenesis and cholesterol transport and functions as an organizer of a number of other critical proteins involved in cell attachment, migration, and signaling. Bacterial and viral pathogens have been shown to attach to this protein on the host cell surface. Mutations in this gene are associated with congenital cataracts in human patients. [provided by RefSeq, Aug 2017]
生物功能	Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2. [UniProt]
产品亮点	Related products: Vimentin antibodies; Vimentin Duos / Panels; Anti-Mouse IgG secondary antibodies; Related news: New antibody panels for Myofibroblasts and CAFs Understanding Your Cells: Choose the right markers SM5-1, a promising immunotherapy for Hepatocellular Carcinoma (HCC) Freeze! Scientists found a way to stop tumor migration New antibody panels and duos for Tumor immune microenvironment Anti-SerpinB9 therapy, a new strategy for cancer therapy
研究领域	Cancer antibody; Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody; Cancer-associated fibroblast antibody; CAF Marker antibody; EMT Study antibody; Mesenchymal Markers antibody; Fibroblast Marker antibody; Muller Cell Marker antibody; Sarcoma Marker antibody
预测分子量	54 kDa
翻译后修饰	Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity). One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33. O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status. S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.

检测图片 (6) Click the Picture to Zoom In

ARG55540 anti-Vimentin antibody ICC/IF image

Immunofluorescence: 100% Methanol fixed (RT, 10 min) HeLa cells stained with ARG55540 anti-Vimentin antibody (green) at 1:800 dilution.

Secondary antibody: ARG55393 Goat anti-Mouse IgG (H+L) antibody (FITC)
ARG55540 anti-Vimentin antibody WB image

Western blot: 20 µg of Colo205, HCT116, HT29, SW480 and SW620 cell lysates stained with ARG55540 anti-Vimentin antibody at 1:2000 dilution.
ARG55540 anti-Vimentin antibody IP image

Immunoprecipitation: HeLa cell lysates were immunoprecipitated and stained with ARG55540 anti-Vimentin antibody.
ARG55540 anti-Vimentin antibody WB image

Western blot: 20 µg of H1299 and A549 cell lysates stained with ARG55540 anti-Vimentin antibody at 1:1000 dilution.
ARG55540 anti-Vimentin antibody ICC/IF image

Immunofluorescence: 100% Methanol fixed (RT, 10 min) HeLa cells stained with ARG55540 anti-Vimentin antibody (green) at 1:800 dilution.

Secondary antibody: ARG55393 Goat anti-Mouse IgG (H+L) antibody (FITC)
ARG55540 anti-Vimentin antibody WB image

Western blot: 20 µg of 293T cell lysate stained with ARG55540 anti-Vimentin antibody at 1:1000 dilution.