ARG44566
anti-WHSC2 / NELF-A antibody
anti-WHSC2 / NELF-A antibody for Flow cytometry,Western blot and Human
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes WHSC2 / NELF-A |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | WHSC2 / NELF-A |
| 抗原物种 | Human |
| 抗原 | Human WHSC2 / NELF-A recombinant protein (aa. sequence: I38-L482). |
| 偶联标记 | Un-conjugated |
| 別名 | NELFA; Negative Elongation Factor Complex Member A; NELF-A; WHSC2; Wolf-Hirschhorn Syndrome Candidate 2 Protein; Negative Elongation Factor A; Wolf-Hirschhorn Syndrome Candidate 2; P/OKcl.15 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose. |
| 抗菌剂 | 4% Trehalose |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | NELFA |
| 全名 | Negative Elongation Factor Complex Member A |
| 背景介绍 | This gene is expressed ubiquitously with higher levels in fetal than in adult tissues. It encodes a protein sharing 93% sequence identity with the mouse protein. Wolf-Hirschhorn syndrome (WHS) is a malformation syndrome associated with a hemizygous deletion of the distal short arm of chromosome 4. This gene is mapped to the 165 kb WHS critical region, and may play a role in the phenotype of the WHS or Pitt-Rogers-Danks syndrome. The encoded protein is found to be capable of reacting with HLA-A2-restricted and tumor-specific cytotoxic T lymphocytes, suggesting a target for use in specific immunotherapy for a large number of cancer patients. This protein has also been shown to be a member of the NELF (negative elongation factor) protein complex that participates in the regulation of RNA polymerase II transcription elongation. [provided by RefSeq, Jul 2008] |
| 生物功能 | Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. [Uniprot] |
| 细胞定位 | Nucleus. [Uniprot] |
| 预测分子量 | 57 kDa |
| 翻译后修饰 | Phosphoprotein. [Uniprot] |
检测图片 (2) Click the Picture to Zoom In
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ARG44566 anti-WHSC2 / NELF-A antibody WB image
Western blot: HeLa, Jurkat and K562 stained with ARG44566 anti-WHSC2 / NELF-A antibody at 0.5 μg/mL dilution.
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ARG44566 anti-WHSC2 / NELF-A antibody FACS image
Flow Cytometry: JK stained with ARG44566 anti-WHSC2 / NELF-A antibody at 1 μg/10^6 cells dilution.
