ARG59525
anti-ZAK antibody
anti-ZAK antibody for ICC/IF,Western blot and Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes ZAK |
|---|---|
| 反应物种 | Ms, Rat |
| 应用 | ICC/IF, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | ZAK |
| 抗原物种 | Human |
| 抗原 | Recombinant fusion protein corresponding to aa. 100-312 of Human ZAK (NP_598407.1). |
| 偶联标记 | Un-conjugated |
| 別名 | AZK; MLK-related kinase; mlklak; EC 2.7.11.25; MLK7; MLK-like mitogen-activated protein triple kinase; MLTK; Human cervical cancer suppressor gene 4 protein; HCCS-4; MLT; MRK; Mitogen-activated protein kinase kinase kinase MLT; pk; Sterile alpha motif- and leucine zipper-containing kinase AZK; Leucine zipper- and sterile alpha motif-containing kinase; Mixed lineage kinase-related kinase |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | Mouse heart and Rat heart | ||||||
| 实际分子量 | 105 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q9ESL4 Mouse Mitogen-activated protein kinase kinase kinase MLT |
|---|---|
| 基因名称 | ZAK |
| 全名 | sterile alpha motif and leucine zipper containing kinase AZK |
| 背景介绍 | This gene is a member of the MAPKKK family of signal transduction molecules and encodes a protein with an N-terminal kinase catalytic domain, followed by a leucine zipper motif and a sterile-alpha motif (SAM). This magnesium-binding protein forms homodimers and is located in the cytoplasm. The protein mediates gamma radiation signaling leading to cell cycle arrest and activity of this protein plays a role in cell cycle checkpoint regulation in cells. The protein also has pro-apoptotic activity. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008] |
| 生物功能 | Stress-activated component of a protein kinase signal transduction cascade. Regulates the JNK and p38 pathways. Pro-apoptotic. Role in regulation of S and G2 cell cycle checkpoint by direct phosphorylation of CHEK2. Isoform 1, but not isoform 2, causes cell shrinkage and disruption of actin stress fibers. Isoform 1 may have role in neoplastic cell transformation and cancer development. Isoform 1, but not isoform 2, phosphorylates histone H3 at 'Ser-28'. [UniProt] |
| 细胞定位 | Cytoplasm. Nucleus. Note=Translocates to the nucleus upon ultraviolet B irradiation. [UniProt] |
| 预测分子量 | 91 kDa |
检测图片 (1) Click the Picture to Zoom In
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ARG59525 anti-ZAK antibody WB image
Western blot: 25 µg of Mouse heart and Rat heart lysates stained with ARG59525 anti-ZAK antibody at 1:3000 dilution.
