ARG55991
anti-acidic Cytokeratin antibody [AE1]
anti-acidic Cytokeratin antibody [AE1] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
概述
产品描述 | Mouse Monoclonal antibody [AE1] recognizes acidic Cytokeratin |
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反应物种 | Hu, Ms, Rat |
应用 | FACS, ICC/IF, IHC-P, WB |
宿主 | Mouse |
克隆 | Monoclonal |
克隆号 | AE1 |
同位型 | IgG1 |
靶点名称 | acidic Cytokeratin |
抗原物种 | Human |
抗原 | Solubilized cytokeratin extract from Human stratum corneum. |
偶联标记 | Un-conjugated |
別名 | KPP; K10; CK-10; BIE; Keratin, type I cytoskeletal 10; Cytokeratin-10; CK10; BCIE; Keratin-10; EHK |
应用说明
应用建议 |
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应用说明 | IHC-P: Antigen Retrieval: Boil tissue section in 10 mM Citrate buffer (pH 6.0) for 10-20 min, followed by cooling at RT for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
形式 | Liquid |
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纯化 | Purification with Protein G. |
缓冲液 | PBS (pH 7.4), 0.05% Sodium azide and 0.1 mg/ml BSA |
抗菌剂 | 0.05% Sodium azide |
稳定剂 | 0.1 mg/ml BSA |
浓度 | 0.2 mg/ml |
存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
数据库连接 | |
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基因名称 | KRT10 |
全名 | keratin 10, type I |
背景介绍 | This gene encodes a member of the type I (acidic) cytokeratin family, which belongs to the superfamily of intermediate filament (IF) proteins. Keratins are heteropolymeric structural proteins which form the intermediate filament. These filaments, along with actin microfilaments and microtubules, compose the cytoskeleton of epithelial cells. Mutations in this gene are associated with epidermolytic hyperkeratosis. This gene is located within a cluster of keratin family members on chromosome 17q21. [provided by RefSeq, Jul 2008] |
细胞定位 | Cytoplasmic |
预测分子量 | 59 kDa |
检测图片 (2) Click the Picture to Zoom In
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ARG55991 anti-acidic Cytokeratin antibody [AE1] IHC-P image
Immunohistochemistry: Human skin stained with ARG55991 anti-acidic Cytokeratin antibody [AE1].
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ARG55991 anti-acidic Cytokeratin antibody [AE1] WB image
Western blot: A431 cell lysate stained with ARG55991 anti-acidic Cytokeratin antibody [AE1].