ARG55991

anti-acidic Cytokeratin antibody [AE1]

anti-acidic Cytokeratin antibody [AE1] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

概述

产品描述 Mouse Monoclonal antibody [AE1] recognizes acidic Cytokeratin
反应物种 Hu, Ms, Rat
应用 FACS, ICC/IF, IHC-P, WB
宿主 Mouse
克隆 Monoclonal
克隆号 AE1
同位型 IgG1
靶点名称 acidic Cytokeratin
抗原物种 Human
抗原 Solubilized cytokeratin extract from Human stratum corneum.
偶联标记 Un-conjugated
別名 KPP; K10; CK-10; BIE; Keratin, type I cytoskeletal 10; Cytokeratin-10; CK10; BCIE; Keratin-10; EHK

应用说明

应用建议
应用 推荐稀释比
FACS0.5 - 1 µg/10^6 cells
ICC/IF1 - 5 µg/ml
IHC-P1 - 5 µg/ml
WB0.5 - 1 µg/ml
应用说明 IHC-P: Antigen Retrieval: Boil tissue section in 10 mM Citrate buffer (pH 6.0) for 10-20 min, followed by cooling at RT for 20 min.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Purification with Protein G.
缓冲液 PBS (pH 7.4), 0.05% Sodium azide and 0.1 mg/ml BSA
抗菌剂 0.05% Sodium azide
稳定剂 0.1 mg/ml BSA
浓度 0.2 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 16661 Mouse KRT10

GeneID: 3858 Human KRT10

GeneID: 450225 Rat KRT10

基因名称 KRT10
全名 keratin 10, type I
背景介绍 This gene encodes a member of the type I (acidic) cytokeratin family, which belongs to the superfamily of intermediate filament (IF) proteins. Keratins are heteropolymeric structural proteins which form the intermediate filament. These filaments, along with actin microfilaments and microtubules, compose the cytoskeleton of epithelial cells. Mutations in this gene are associated with epidermolytic hyperkeratosis. This gene is located within a cluster of keratin family members on chromosome 17q21. [provided by RefSeq, Jul 2008]
细胞定位 Cytoplasmic
预测分子量 59 kDa

检测图片 (2) Click the Picture to Zoom In

  • ARG55991 anti-acidic Cytokeratin antibody [AE1] IHC-P image

    Immunohistochemistry: Human skin stained with ARG55991 anti-acidic Cytokeratin antibody [AE1].

  • ARG55991 anti-acidic Cytokeratin antibody [AE1] WB image

    Western blot: A431 cell lysate stained with ARG55991 anti-acidic Cytokeratin antibody [AE1].